MORPHINE MODULATES CATHEPSIN-B AND CATHEPSIN-L ACTIVITY IN ISOLATED GLOMERULI AND MESANGIAL CELLS

被引：2

作者：

SINGHAL, PC ^{[1
]}

SAGAR, S ^{[1
]}

GIBBONS, N ^{[1
]}

机构：

[1] ALBERT EINSTEIN COLL MED,BRONX,NY

来源：

INFLAMMATION | 1995年 / 19卷 / 01期

关键词：

D O I：

10.1007/BF01534381

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Altered matrix degradation may be playing a role in the development of initial mesangial expansion and subsequent glomerulosclerosis in persons with heroin abuse. We studied whether morphine, a metabolite of heroin, had any effect on lysosomal content of cathepsin B and L in mesangial cells. Morphine (10(-6) M) increased (P < 0.01) mesangial cathepsin B and L activity (control, 22.1 +/- 2.2 vs. morphine, 31.4 +/- 1.4 mol NMec/mu g protein, N = 5). Morphine (10(-6) M) also increased (P < 0.01) glomerular cathepsin B and L activity (control, 0.1 +/- 0.01 vs. morphine, 2.2 +/- 0.2 pmol NMec/mu g protein, N = 3). This effect of morphine occurred in a dose-dependent manner. These results suggest that morphine enhances cathepsin B and L activity in mesangial cells and isolated glomeruli. This effect of morphine may enhance capacity of mesangial cells to degrade increased amount of mesangial macromolecules.

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页码：67 / 73

页数：7

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