BIOASSAY OF BOVINE INTERLEUKIN-1-LIKE ACTIVITY

被引：9
|
作者：
WINSTANLEY, FP [1 ]
ECKERSALL, PD [1 ]
机构：
[1] UNIV GLASGOW, SCH VET, DEPT VET BIOCHEM, GLASGOW G61 1QH, SCOTLAND
来源：
RESEARCH IN VETERINARY SCIENCE | 1992年 / 52卷 / 03期
关键词：
D O I：
10.1016/0034-5288(92)90024-V
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A bioassay for bovine interleukin-1 (IL1) activity is described. The assay is based on the IL1-stimulated proliferation of a mouse T-lymphocyte cell line, D10(N4)M. Bovine mononuclear cells stimulated with lipopolysaccharide produce an interleukin-1-like activity which stimulated the growth of the D10(N4)M cell line in a dose-dependent manner. The stimulatory activity was neutralised by a combination of both anti-human IL1-alpha and anti-human IL1-beta sera. The quantity of IL1-like activity released from the mononuclear cells increased asymptotically with increasing lipopolysaccharide dose.
引用
收藏
页码：273 / 276
页数：4
相关论文
共 50 条
  • [41] A FACTOR WITH INTERLEUKIN-1-LIKE ACTIVITY IS PRODUCED BY PERITONEAL-EXUDATE CELLS FROM THE FROG, XENOPUS-LAEVIS
    WATKINS, D
    PARSONS, SV
    COHEN, N
    DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1987, 11 (02): : 455 - 455
  • [42] MALIGNANT-TUMOR CELL-LINES PRODUCE INTERLEUKIN-1-LIKE FACTOR
    MIYAUCHI, S
    MOROYAMA, T
    KYOIZUMI, S
    ASAKAWA, JI
    OKAMOTO, T
    TAKADA, K
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY, 1988, 24 (08): : 753 - 758
  • [43] SPONTANEOUS PRODUCTION OF INTERLEUKIN-1-LIKE FACTORS FROM PREGNANT RABBIT UTERINE CERVIX
    ITO, A
    HIRO, D
    OJIMA, Y
    MORI, Y
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1988, 159 (01) : 261 - 265
  • [44] COLLAGENASE-STIMULATING AND PGE2-STIMULATING ACTIVITY (INTERLEUKIN-1-LIKE) AND INHIBITOR(S) IN HUMAN-URINE
    DAYER, JM
    BALAVOINE, JF
    CRUCHAUD, A
    DEROCHEMONTEIX, B
    JOURNAL OF LEUKOCYTE BIOLOGY, 1985, 37 (06) : 693 - 693
  • [45] ENDOTOXIN-ASSOCIATED PROTEIN - INTERLEUKIN-1-LIKE ACTIVITY ON SERUM AMYLOID-A SYNTHESIS AND LYMPHOCYTE-T ACTIVATION
    JOHNS, MA
    SIPE, JD
    MELTON, LB
    STROM, TB
    MCCABE, WR
    INFECTION AND IMMUNITY, 1988, 56 (06) : 1593 - 1601
  • [46] THE SAME CLONED MURINE B-LYMPHOMA CELL-LINES CAN BE SELECTIVELY INDUCED TO RELEASE EITHER INTERLEUKIN-1-LIKE OR INTERLEUKIN-2-LIKE FACTOR ACTIVITY
    WALKER, EB
    LEEMHUIS, T
    LYMPHOKINE RESEARCH, 1987, 6 (02): : 71 - 81
  • [47] ISOLATION AND CHARACTERIZATION OF A PRIMITIVE INTERLEUKIN-1-LIKE PROTEIN FROM AN INVERTEBRATE, ASTERIAS-FORBESI
    BECK, G
    HABICHT, GS
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) : 7429 - 7433
  • [48] ISOLATION AND PARTIAL CHARACTERIZATION OF AN INTERLEUKIN-1-LIKE FACTOR FROM RAT TESTIS INTERSTITIAL FLUID
    GUSTAFSSON, K
    SODER, O
    POLLANEN, P
    RITZEN, EM
    JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 1988, 14 (02) : 139 - 150
  • [49] INTERLEUKIN-1-LIKE ACTIVITY CONSTITUTIVELY GENERATED BY HODGKIN DERIVED CELL-LINES .1. MEASUREMENT IN A HUMAN-LYMPHOCYTE COSTIMULATOR ASSAY
    KORTMANN, C
    BURRICHTER, H
    MONNER, D
    JAHN, G
    DIEHL, V
    PETER, HH
    IMMUNOBIOLOGY, 1984, 166 (03) : 318 - 333
  • [50] OPIOID INDUCTION OF IMMUNOREACTIVE INTERLEUKIN-1 IN MYTILUS-EDULIS AND HUMAN IMMUNOCYTES - AN INTERLEUKIN-1-LIKE SUBSTANCE IN INVERTEBRATE NEURAL TISSUE
    STEFANO, GB
    SMITH, EM
    HUGHES, TK
    JOURNAL OF NEUROIMMUNOLOGY, 1991, 32 (01) : 29 - 34
12345