RAPID IDENTIFICATION AND ISOLATION OF ZYGOTES AND THE KINETICS OF MATING IN SACCHAROMYCES-CEREVISIAE

被引：3
|
作者：
BLOCK, DE
SRIENC, F
机构：
[1] UNIV MINNESOTA,DEPT CHEM ENGN & MAT SCI,MINNEAPOLIS,MN 55455
[2] UNIV MINNESOTA,INST ADV STUDIES BIOL PROCESS TECHNOL,MINNEAPOLIS,MN 55455
来源：
BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 02期
关键词：
D O I：
10.1007/BF00159978
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Flow cytometry has been used in the selection of fusion products of the yeast Saccharomyces cerevisiae. Parental cells of opposite mating type were stained with different fluorescent dyes, permitting rapid identification of zygotes from natural matings based on dual-color flow cytometry. This procedure was then used to study the kinetics of mating in yeast and the physical and biological parameters that affect these kinetics, such as cell concentration, parental ratios, and parental strain growth rate.
引用
收藏
页码：95 / 100
页数：6
相关论文
共 50 条
  • [31] ISOLATION AND CHARACTERIZATION OF GUANINE AUXOTROPHS IN SACCHAROMYCES-CEREVISIAE
    GARDNER, WJE
    WOODS, RA
    CANADIAN JOURNAL OF MICROBIOLOGY, 1979, 25 (03) : 380 - 389
  • [32] ISOLATION AND CHARACTERIZATION OF GAMMA DNA OF SACCHAROMYCES-CEREVISIAE
    CRAMER, JH
    BHARGAVA, MM
    HALVORSON, HO
    JOURNAL OF MOLECULAR BIOLOGY, 1972, 71 (01) : 11 - +
  • [33] ISOLATION AND CHARACTERIZATION OF SULFITE MUTANTS OF SACCHAROMYCES-CEREVISIAE
    XU, X
    WIGHTMAN, JD
    GELLER, BL
    AVRAM, D
    BAKALINSKY, AT
    CURRENT GENETICS, 1994, 25 (06) : 488 - 496
  • [34] ISOLATION OF SACCHAROMYCES-CEREVISIAE TRP3
    PALUH, JL
    ZALKIN, H
    JOURNAL OF BACTERIOLOGY, 1983, 153 (01) : 345 - 349
  • [35] ISOLATION AND CHARACTERIZATION OF AMINOPEPTIDASE MUTANTS OF SACCHAROMYCES-CEREVISIAE
    TRUMBLY, RJ
    BRADLEY, G
    JOURNAL OF BACTERIOLOGY, 1983, 156 (01) : 36 - 48
  • [36] ISOLATION OF SECRETORY VESICLES FROM SACCHAROMYCES-CEREVISIAE
    HOLCOMB, CL
    ETCHEVERRY, T
    SCHEKMAN, R
    ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) : 328 - 334
  • [37] ISOLATION OF COATED VESICLES FROM SACCHAROMYCES-CEREVISIAE
    MUELLER, SC
    BRANTON, D
    JOURNAL OF CELL BIOLOGY, 1982, 95 (02): : A389 - A389
  • [38] ISOLATION OF CENIX AND CENXII FROM SACCHAROMYCES-CEREVISIAE
    WUSTINGER, K
    SPEVAK, W
    NUCLEIC ACIDS RESEARCH, 1993, 21 (14) : 3321 - 3321
  • [39] ISOLATION AND CHARACTERIZATION OF THE NUCLEI FROM SACCHAROMYCES-CEREVISIAE
    KAMIMURA, K
    TSUCHIYA, E
    MIYAKAWA, T
    FUKI, S
    HIRATA, A
    CURRENT MICROBIOLOGY, 1981, 6 (03) : 175 - 180
  • [40] A RAPID AND TRANSIENT INCREASE OF CELLULAR CA-2+ IN RESPONSE TO MATING PHEROMONE IN SACCHAROMYCES-CEREVISIAE
    TACHIKAWA, T
    MIYAKAWA, T
    TSUCHIYA, E
    FUKUI, S
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1987, 51 (04): : 1209 - 1210
12345