RAPID IDENTIFICATION AND ISOLATION OF ZYGOTES AND THE KINETICS OF MATING IN SACCHAROMYCES-CEREVISIAE

被引：3
|
作者：
BLOCK, DE
SRIENC, F
机构：
[1] UNIV MINNESOTA,DEPT CHEM ENGN & MAT SCI,MINNEAPOLIS,MN 55455
[2] UNIV MINNESOTA,INST ADV STUDIES BIOL PROCESS TECHNOL,MINNEAPOLIS,MN 55455
来源：
BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 02期
关键词：
D O I：
10.1007/BF00159978
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Flow cytometry has been used in the selection of fusion products of the yeast Saccharomyces cerevisiae. Parental cells of opposite mating type were stained with different fluorescent dyes, permitting rapid identification of zygotes from natural matings based on dual-color flow cytometry. This procedure was then used to study the kinetics of mating in yeast and the physical and biological parameters that affect these kinetics, such as cell concentration, parental ratios, and parental strain growth rate.
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页码：95 / 100
页数：6
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