EVIDENCE FOR 2 STRUCTURAL GENES FOR ALKALINE-PHOSPHATASE IN BACILLUS-SUBTILIS

被引：79
作者：
HULETT, FM
BOOKSTEIN, C
JENSEN, K
机构：
[1] Lab. for Molecular Biology, University of Illinois, Chicago
来源：
JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 02期
关键词：
D O I：
10.1128/jb.172.2.735-740.1990
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two secreted alkaline phosphatase proteins were purified from cultures of Bacillus subtilis JH646MS. The two proteins showed slight differences in subunit molecular weight, substrate specificity, and charge characteristics. A total of 62% of the first 22 amino-terminal amino acids were identical. Both sequences showed conservation of structural features identified in Escherichia coli and human alkaline phosphatases. One alkaline phosphatase was a monomer and the other was a dimer. Southern analysis of genomic DNA with degenerative oligomers based on the amino acid sequences suggest that there are two structural genes for alkaline phosphatase in the genome of B. subtilis.
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页码：735 / 740
页数：6
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