PURIFICATION OF HUMAN LIVER FUMARYLACETOACETASE USING IMMUNOAFFINITY CHROMATOGRAPHY

被引：2

作者：

VANFAASSEN, H ^{[1
]}

VANDENBERG, IET ^{[1
]}

BERGER, R ^{[1
]}

机构：

[1] UNIV GRONINGEN,DEPT PEDIAT,10 BLOEMSINGEL,9712 KZ GRONINGEN,NETHERLANDS

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 20卷 / 04期

关键词：

Fumarylacetoacetase; Hydrolase; Immunoaffinity chromatography; Protein/enzyme purification; Tyrosine; Tyrosinemia type I;

D O I：

10.1016/0165-022X(90)90093-R

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described to purify fumarylacetoacetase from crude human liver extracts using immunoaffinity chromatography. Immobilized rabbit antibodies specific for beef liver fumarylacetoacetase were used as an immunoadsorbent. With this rapid and specific procedure human liver fumarylacetoacetase could be purified to apparent homogeneity. The molecular weight of native human liver fumarylacetoacetase is approximately 83000 as estimated by gel filtration. The two subunits have a molecular weight of approximately 41000, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Purified human liver fumarylacetoacetase has a broad pH optimum with a maximum at pH 7.2 and a Km = 2.1 μM towards fumarylacetoacetate. © 1990.

引用

页码：317 / 324

页数：8

共 50 条

[1] PURIFICATION OF HUMAN ERYTHROPOIETIN BY IMMUNOAFFINITY CHROMATOGRAPHY
MARTINELLI, RA
SUE, JM
SYTKOWSKI, AJ
CLINICAL RESEARCH, 1985, 33 (02): : A349 - A349
[2] PURIFICATION OF HUMAN-ERYTHROCYTE TRANSGLUTAMINASE BY IMMUNOAFFINITY CHROMATOGRAPHY
LEE, KN
BIRCKBICHLER, PJ
FESUS, L
PREPARATIVE BIOCHEMISTRY, 1986, 16 (04): : 321 - 335
[3] PURIFICATION OF SELENOPROTEIN-P FROM HUMAN PLASMA USING IMMUNOAFFINITY CHROMATOGRAPHY
AKESSON, B
BELLEW, T
BURK, RF
FASEB JOURNAL, 1991, 5 (04): : A714 - A714
[4] A LARGE-SCALE PURIFICATION OF BETA-GLUCURONIDASE FROM HUMAN LIVER BY IMMUNOAFFINITY CHROMATOGRAPHY
HO, KJ
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 1991, 14 (03) : 296 - 305
[5] THE PURIFICATION OF HUMAN THYROID-STIMULATING HORMONE BY IMMUNOAFFINITY CHROMATOGRAPHY
JACK, GW
BLAZEK, R
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 1987, 39 (01) : 1 - 9
[6] PURIFICATION OF THE HUMAN-ERYTHROCYTE NUCLEOSIDE TRANSPORTER BY IMMUNOAFFINITY CHROMATOGRAPHY
KWONG, FYP
DAVIES, A
TSE, CM
YOUNG, JD
HENDERSON, PJF
BALDWIN, SA
BIOCHEMICAL JOURNAL, 1988, 255 (01) : 243 - 249
[7] PURIFICATION OF RECOMBINANT HUMAN TRANSCRIPTION FACTOR IIB BY IMMUNOAFFINITY CHROMATOGRAPHY
THOMPSON, NE
BURGESS, RR
PROTEIN EXPRESSION AND PURIFICATION, 1994, 5 (05) : 468 - 475
[8] Immunoaffinity Chromatography for Protein Purification and Analysis
Sharmeen, Sadia
Suh, Kyungah
Kyei, Isaac
Jones, Jacob
Olupathage, Harshana
Campbell, Avery
Hage, David S.
CURRENT PROTOCOLS, 2023, 3 (08):
[9] PURIFICATION OF GPI ANCHORS BY IMMUNOAFFINITY CHROMATOGRAPHY
HEYWOOD, SP
HOOPER, NM
BIOCHEMICAL SOCIETY TRANSACTIONS, 1995, 23 (01) : S101 - S101
[10] PURIFICATION OF MILK CATALASE BY IMMUNOAFFINITY CHROMATOGRAPHY
ITO, O
KAMATA, S
KAKIICHI, N
SUZUKI, Y
HAYASHI, M
UCHIDA, K
JOURNAL OF FOOD SCIENCE, 1990, 55 (04) : 1172 - 1173

← 1 2 3 4 5 →