MU-OPIOID RECEPTOR-BINDING IN INTACT SH-SY5Y NEUROBLASTOMA-CELLS

被引：15
|
作者：
TOLL, L
机构：
[1] Life Science Division, SRI International, Menlo Park, CA 94025
来源：
EUROPEAN JOURNAL OF PHARMACOLOGY | 1990年 / 176卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Neuroblastoma; SH-SY5Y; [!sup]3[!/sup]H]CTOP; μ Opioid receptors;
D O I：
10.1016/0014-2999(90)90530-J
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
In order to determine affinities at the μ opioid receptor binding was conducted to intact SH-SY5Y neuroblastoma cells using the μ-selective ligand [3H][H-D-Phe-Cys-Tyr-D-Trp-Orn-Thr-Pen-Thr-NH2] ([3H]CTOP). Binding appeared to be to a single receptor site, and a single state of the μ receptor. Under intact cell conditions, some but not all μ agonists display low affinity binding, while antagonists maintain high affinity for the μ receptor. These studies indicate the usefulness of [3H]CTOP for the determination of affinities at the μ receptor under physiological conditions. © 1990.
引用
收藏
页码：213 / 217
页数：5
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