ACTIVATION OF LYT-2 ASSOCIATED WITH DISTANT UPSTREAM INSERTION OF AN SL3-3 PROVIRUS

被引：2
作者：
ANSON, DS [1 ]
CLARKIN, K [1 ]
HYMAN, R [1 ]
机构：
[1] SALK INST, DEPT CANC BIOL, POB 85800, SAN DIEGO, CA 92186 USA
来源：
IMMUNOGENETICS | 1992年 / 36卷 / 01期
关键词：
D O I：
10.1007/BF00209286
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Two Lyt-2+ mutants of the T-cell lymphoma SL12.4.10 were selected by fluorescence activated cell sorting. Both mutants expressed Lyt-2 (CD8 alpha-chain) but not Lyt-3 (CD8 beta-chain). Derivatives of one Lyt-2+ mutant that expressed Lyt-3 could be isolated by sorting for Lyt-3+ cells. Southern blotting analysis indicated that both mutants had structural rearrangements within or immediately 3' of the Lyt-3 gene, accompanied by demethylation of at least one Hpa II site within the Lyt-2 gene. Gene cloning analysis of one mutant demonstrated that the structural rearrangement was due to insertion of an SL3-3 provirus 35 kb 5' to the Lyt-2 gene. It is likely that Lyt-2 gene activation is a direct or indirect consequence of proviral insertion at this site.
引用
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页码：3 / 14
页数：12
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