SUBUNIT STRUCTURE OF ALPHA-SATELLITE DNA CONTAINING CHROMATIN FROM AFRICAN-GREEN MONKEY CELLS

被引：59

作者：

FITTLER, F

ZACHAU, HG

机构：

[1] Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München, 8000 München 2

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 7卷 / 01期

关键词：

D O I：

10.1093/nar/7.1.1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

α-satellite DNA containing chromatin from African green monkey cells (CV-1 cells) has been used to study the question whether or not nucleosomes are arranged in phase with the 172 bp repeat unit of the satellite DNA. Digestion experiments with DNAase II led us to exclude a simple phase relationship between the nucleosomal and the satellite DNA repeats. Digestion of CV-1 nuclei with micrococcal nuclease and endogenous nuclease(s) produced a series of sharp bands in the satellite DNA register over a background of heterogeneous length fragments. This observation is explained by a preferential cleavage of certain nucleotide sequences by these nucleases and is not in contradiction to our conclusion that a simple phase relationship does not exist. © 1979 Information Retrieval Limited.

引用

页码：1 / 13

页数：13

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