INTRACELLULAR PANCREATIC B-CELL SEROTONIN AND DYNAMICS OF INSULIN RELEASE

被引：11
|
作者：
PULIDO, OM
BENCOSME, SA
DEBOLD, ML
DEBOLD, AJ
机构：
[1] HOTEL DIEU HOSP,DEPT PATHOL,KINGSTON K7L 3H6,ONTARIO,CANADA
[2] QUEENS UNIV,DEPT PATHOL,KINGSTON K7L 3N6,ONTARIO,CANADA
来源：
DIABETOLOGIA | 1978年 / 15卷 / 03期
关键词：
fluorescence microscopy; Insulin; L-aromatic amino acid decarboxylase; monoamine oxidase; pancreatic B cells; serotonin;
D O I：
10.1007/BF00421239
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The role of intracellular pancreatic B cell serotonin in the dynamics of insulin release was investigated in an in situ perfused rat pancreas preparation. Animals were pretreated with 5-hydroxytryptophan (5-HTP) to increase the intracellular levels of serotonin (5-HT) as shown by fluorescence histochemistry. Despite a clear induction of intracellular 5-HT fluorescence in pancreatic islets neither the pattern nor the total amount of insulin released were significantly modified after perfusion with either glucose or tolbutamide. However, the L-amino acid decarboxylase inhibitor, RO 4-4602, significantly decreased both phases of glucose-mediated insulin release in normal animals as well as in those receiving 5-HTP. © 1978 Springer-Verlag.
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页码：197 / 204
页数：8
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