SUPEROXIDE FROM GLUCOSE-OXIDASE OR FROM NITROBLUE TETRAZOLIUM

被引：57

作者：

LIOCHEV, SI ^{[1
]}

FRIDOVICH, I ^{[1
]}

机构：

[1] DUKE UNIV,MED CTR,DEPT BIOCHEM,DURHAM,NC 27710

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 318卷 / 02期

关键词：

NITROBLUE TETRAZOLIUM; O-2(-) PRODUCTION BY GLUCOSE OXIDASE; NBT REDUCTION BY SUPEROXIDE; INHIBITION OF NBT REDUCTION BY OXYGEN; INHIBITION OF NBT REDUCTION BY CYTOCHROME C; REDUCTION OF NBT BY GLUCOSE OXIDASE;

D O I：

10.1006/abbi.1995.1247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glucose oxidase reduces nitroblue tetrazolium, or ferricytochrome c, faster anaerobically thari aerobically. This result is inconsistent with the conclusion that GO(2) can reduce O-2 to O-2(-) which is then responsible for the reduction of NET and of cytochrome c. Nevertheless, the aerobic reductions are partially inhibitable by superoxide dismutase. A scheme of reactions is proposed which explains why these electron acceptors cause an O-2(-) production which does not occur in their absence when O-2 is the sole electron acceptor. (C) 1995 Academic Press, Inc.

引用

页码：408 / 410

页数：3

共 13 条

[1]

ALBEKAIRI AM, 1994, BIOCHEM MOL BIOL INT, V34, P233

[2] SUPEROXIDE ANION INVOLVEMENT IN NBT REDUCTION CATALYZED BY NADPH-CYTOCHROME RHO-450 REDUCTASE - PITFALL [J].