DETECTION OF AAC(6')-I GENES IN AMIKACIN-RESISTANT ACINETOBACTER SPP BY PCR

被引：32

作者：

PLOY, MC

GIAMARELLOU, H

BOURLIOUX, P

COURVALIN, P

LAMBERT, T

机构：

[1] INST PASTEUR,CNRS,UNITE AGENTS ANTIBACTERIENS,EP J0058,F-75724 PARIS 15,FRANCE

[2] CTR ETUD PHARMACEUT,F-92296 CHATENAY MALABRY,FRANCE

[3] HOSP LAIKO,GR-11527 ATHENS,GREECE

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1994年 / 38卷 / 12期

关键词：

D O I：

10.1128/AAC.38.12.2925

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The distribution of aac(6')-I genes in 62 strains of Acinetobacter spp. resistant to amikacin, netilmicin, and tobramycin and susceptible to gentamicin, a phenotype compatible with synthesis of an AAC(6')-I enzyme, was studied by PCR and by DNA hybridization. Both methods gave similar results. Among the 51 Acinetobacter baumannii strains, aac(6')-Ib was found in 19 isolates and aac(6')-Ih was found in the remaining strains. The aac(6')-Ig gene was present in all 10 A. haemolyticus strains studied and was detected only in this species. A pair of degenerate oligonucleotides complementary to conserved regions of aac(6')-Ic, -Id, -If, -Ig, and -Ih enabled detection of these genes and also of aac(6')-Ij, recently recognized in Acinetobacter sp. strain 13.

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页码：2925 / 2928

页数：4

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