PURIFICATION AND CHARACTERIZATION OF BETA-FRUCTOFURANOSIDASE FROM ASPERGILLUS-JAPONICUS TIT-KJ1

被引：27

作者：

DUAN, KJ

SHEU, DC

CHEN, JS

机构：

[1] Department of Bioengineering, Tatung Institute of Technolgoy, Taipei 10451

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1993年 / 57卷 / 11期

关键词：

D O I：

10.1271/bbb.57.1811

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A beta-fructofuranosidase (EC 3.2.1.26) was purified to homogeneity from Aspergillus japonicus TIT-KJ1. The enyme had an optimum pH for activity of 5.4 and pH stability at 7.0-8.4. The optimum temperature at pH 5.4 was 60-degrees-C. The enzyme had a molecular weight of 236,000 with two subunits and an isoelectric point of pH 4.0. The enzyme was inactivated by 5 mM Hg2+ and Ag+. The enzyme had a high transfructosylating activity. Treatment of 50% (w/v) sucrose with the enzyme under optimum conditions afforded more than 55% fructooligosaccharides.

引用

页码：1811 / 1815

页数：5

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