CRUCIAL ROLE OF PYROPHOSPHATE IN THE AMINOACYLATION OF ESCHERICHIA-COLI TRANSFER RNA(PHE) BY YEAST PHENYLALANYL-TRANSFER RNA-SYNTHETASE

被引：8

作者：

KHVOROVA, AM ^{[1
]}

MOTORIN, YA ^{[1
]}

WOLFSON, AD ^{[1
]}

机构：

[1] AN BAKH BIOCHEM INST, LENINSKY PR 33, MOSCOW 117071, RUSSIA

来源：

FEBS LETTERS | 1992年 / 311卷 / 02期

关键词：

PHENYLALANYL-TRANSFER RNA SYNTHETASE; PYROPHOSPHATE; TRANSFER RNA; ENZYME INACTIVATION;

D O I：

10.1016/0014-5793(92)81385-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rapid inactivation of the yeast phenylalanyl-tRNA synthetase in the course of aminoacylation of the heterologous E. coli tRNA(Phe) is observed. This inactivation occurs due to the formation of the tight complex of the enzyme with the pyrophosphate formed during the aminoacylation reaction. This complex is shown to be the normal intermediate of the reaction. Possible inactivation mechanism and correlation between structural differences of yeast and E. coli tRNAs(Phe) with the changes in the enzymatic mechanism of aminoacylation are discussed.

引用

页码：139 / 142

页数：4

共 16 条

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