AGE, DIETARY SELENIUM AND QUANTITY OF 7,12-DIMETHYLBENZ(A)ANTHRACENE INFLUENCE THE INVIVO OCCURRENCE OF RAT MAMMARY DNA ADDUCTS

被引:24
作者
LIU, JZ
MILNER, JA
机构
[1] PENN STATE UNIV, DEPT NUTR, University Pk, PA 16802 USA
[2] PENN STATE UNIV, GRAD PROGRAM NUTR, University Pk, PA 16802 USA
关键词
SELENIUM; MAMMARY DNA-ADDUCTS; 7,12-DIMETHYLBENZ(A)ANTHRACENE; RATS; P-32-POSTLABELING;
D O I
10.1093/jn/122.7.1361
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The present studies determined the impact of age, dietary selenium and 7,12-dimethylbenz(a)anthracene (DMBA) dosage on the occurrence of DMBA-DNA adducts in rat mammary tissue. Diets formulated to contain selenium, as sodium selenite, at 0.1 (control) or 2.0 mg/kg were fed for 2 wk before DMBA treatment. Food intake and weight gain were not influenced by selenium intake. Anti- and syn-dihydrodiol epoxide adducts reached maximum binding by 24 and 36 h, respectively, after treatment with DMBA. Consumption of the diet containing 2.0 mg Se/kg inhibited the appearance of both anti- and syn-adducts by approximately 50% compared with controls. The occurrence of DMBA-DNA adducts correlated with a dosage of DMBA from 5 to 50 mg/kg body wt (r greater-than-or-equal-to 0.95). The ability of supplemental selenite to lower DMBA binding to mammary cell DNA increased as the quantity of the carcinogen administered increased. DMBA-DNA binding was found to increase with the increasing age of the rat. Nevertheless, dietary selenium supplementation was effective in reducing DMBA binding to DNA in all ages examined. These data confirmed the ability of dietary selenium to inhibit the in vivo metabolism of DMBA under a variety of conditions.
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页码:1361 / 1368
页数:8
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