A SIMPLE SPECTROPHOTOMETRIC SCANNING PROCEDURE FOR QUANTITATIVE ESTIMATION OF PROTEIN IN POLYACRYLAMIDE GELS

被引：14
作者：
DRAVID, AR
FREDEN, H
LARSSON, S
机构：
[1] Institute of Neurobiology, Faculty of Medicine, University of Göteborg, Göteborg
来源：
JOURNAL OF CHROMATOGRAPHY | 1969年 / 41卷 / 01期
关键词：
D O I：
10.1016/0021-9673(64)80097-2
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple and rapid spectrophotometric procedure for the scanning of polyacrylamide gels in ultraviolet light is described. It is shown that the absorption of the albumin band in acrylamide gel is proportional to the quantity of albumin subjected to electrophoresis and that the procedure can be applied to the study of serum and tissue protein separations in polyacrylamide gels. Some of the advantages of this method over other currently used methods are indicated. © 1969.
引用
收藏
页码：53 / &
相关论文
共 12 条
[1]   A METHOD FOR DETERMINATION OF RELATIVE SPECIFIC RADIOACTIVITY OF (3H) PROTEINS IN ACRYLAMIDE GEL [J].
CAIN, DF ;
PITNEY, RE .
ANALYTICAL BIOCHEMISTRY, 1968, 22 (01) :11-&
[2]   DISC ELECTROPHORESIS .2. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS [J].
DAVIS, BJ .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1964, 121 (A2) :404-&
[3]   APPLICATION OF DISC ELECTROPHORESIS TO STUDY OF BRAIN PROTEINS [J].
GANDINI, S ;
HYDEN, H .
JOURNAL OF CHROMATOGRAPHY, 1968, 32 (04) :715-&
[4]   A SPECTROPHOTOMETER ATTACHMENT FOR SCANNING POLYACRYLAMIDE GELS [J].
GRESSEL, J ;
WOLOWELSKY, J .
ANALYTICAL BIOCHEMISTRY, 1968, 24 (01) :157-+
[5]   A SIMPLE IN-VIAL COMBUSTION METHOD FOR ASSAY OF HYDROGEN-3 CARBON-14 AND SULFUR-35 IN BIOLOGICAL BIOCHEMICAL AND ORGANIC MATERIALS [J].
GUPTA, GN .
ANALYTICAL CHEMISTRY, 1966, 38 (10) :1356-&
[6]   ELECTROPHORESIS OF HISTONES IN POLYACRYLAMIDE GEL AND THEIR QUANTITATIVE DETERMINATION [J].
JOHNS, EW .
BIOCHEMICAL JOURNAL, 1967, 104 (01) :78-&
[7]   FRACTIONATION OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC ACID BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
LOENING, UE .
BIOCHEMICAL JOURNAL, 1967, 102 (01) :251-+
[8]   A STUDY OF SPECIFIC BRAIN PROTEINS ON SEMI-MICRO SCALE [J].
MCEWEN, BS ;
HYDEN, H .
JOURNAL OF NEUROCHEMISTRY, 1966, 13 (09) :823-+
[9]   A SOLUBLE PROTEIN CHARACTERISTIC OF NERVOUS SYSTEM [J].
MOORE, BW .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 19 (06) :739-&
[10]  
NEUHOFF V, 1968, Z PHYSIOL CHEM, V349, P795
12