EXPRESSION OF HUMAN INTERLEUKIN-10 IN ESCHERICHIA-COLI-CELLS

被引：1
|
作者：
PTITSYN, LR [1 ]
ALTMAN, IB [1 ]
GUROV, MV [1 ]
KURKIN, AN [1 ]
机构：
[1] BIOTEKHNOL JOINT STOCK CO,INST BIOTECHNOL,DEPT MOLEC BIOL,MOSCOW,RUSSIA
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 1995年 / 119卷 / 03期
关键词：
INTERLEUKIN-10; GENE SYNTHESIS; PRODUCER STRAIN;
D O I：
10.1007/BF02445847
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The human interleukin-10 gene has been synthesized by the chemical enzymatic method. Vectors for cytoplasmic and periplasmic expression of recombinant interleukin-10 have been obtained in E. coli cells. A high level of protein expression was found to be characteristic of only recombinant strains producing interleukin-10 as ''fused'' protein (fused with the N-terminal fragment of interleukin-3).
引用
收藏
页码：316 / 319
页数：4
相关论文
共 50 条
  • [31] Role of interleukin-10 and interleukin-10 receptor in systemic lupus erythematosus
    Peng, Hui
    Wang, Wei
    Zhou, Mo
    Li, Rui
    Pan, Hai-Feng
    Ye, Dong-Qing
    CLINICAL RHEUMATOLOGY, 2013, 32 (09) : 1255 - 1266
  • [32] Role of interleukin-10 and interleukin-10 receptor in systemic lupus erythematosus
    Hui Peng
    Wei Wang
    Mo Zhou
    Rui Li
    Hai-Feng Pan
    Dong-Qing Ye
    Clinical Rheumatology, 2013, 32 : 1255 - 1266
  • [33] Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
    Wang, Shui-Bin
    Deng, Yu-Qin
    Ren, Jie
    Xiao, Bo-Kui
    Liu, Zheng
    Tao, Ze-Zhang
    BMC IMMUNOLOGY, 2014, 15
  • [34] Suppression of interleukin-10 release from human periodontal ligament cells by interleukin-1β in vitro
    Deschner, J
    Arnold, B
    Kage, A
    Zimmermann, B
    Kanitz, V
    Bernimoulin, JP
    ARCHIVES OF ORAL BIOLOGY, 2000, 45 (02) : 179 - 183
  • [35] Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
    Shui-Bin Wang
    Yu-Qin Deng
    Jie Ren
    Bo-Kui Xiao
    Zheng Liu
    Ze-Zhang Tao
    BMC Immunology, 15
  • [36] High Interleukin-10 Expression in Type 2 T Helper Cells in Septic Patients
    Wu, Huang-Pin
    Chu, Chien-Ming
    Kao, Kuo-Chin
    Huang, Shu-Huan
    Chuang, Duen-Yau
    IMMUNOLOGICAL INVESTIGATIONS, 2017, 46 (04) : 385 - 394
  • [37] Expression of human leukocyte antigen-G and its correlation with interleukin-10 expression in cervical carcinoma
    Yoon, B. S.
    Kim, Y. T.
    Kim, J. W.
    Kim, S. H.
    Kim, J. H.
    Kim, S. W.
    INTERNATIONAL JOURNAL OF GYNECOLOGY & OBSTETRICS, 2007, 98 (01) : 48 - 53
  • [38] INTERLEUKIN-10 MESSENGER-RIBONUCLEIC-ACID IN HUMAN PLACENTA - IMPLICATIONS OF A ROLE FOR INTERLEUKIN-10 IN FETAL ALLOGRAFT PROTECTION
    CADET, P
    RADY, PL
    TYRING, SK
    YANDELL, RB
    HUGHES, TK
    AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1995, 173 (01) : 25 - 29
  • [39] PRESENCE OF INTERLEUKIN-10 TRANSCRIPTS IN HUMAN PITUITARY AND HYPOTHALAMUS
    RADY, PL
    SMITH, EM
    CADET, P
    OPP, MR
    TYRING, SK
    HUGHES, TK
    CELLULAR AND MOLECULAR NEUROBIOLOGY, 1995, 15 (02) : 289 - 296
  • [40] Transforming growth factor-β inhibits interleukin-10 synthesis by human monocytic cells
    Suberville, S
    Bellocq, A
    Peguillet, I
    Lantz, O
    Stordeur, P
    Fouqueray, B
    Baud, L
    EUROPEAN CYTOKINE NETWORK, 2001, 12 (01) : 141 - 146
12345