EXPRESSION OF HUMAN INTERLEUKIN-10 IN ESCHERICHIA-COLI-CELLS

被引：1
|
作者：
PTITSYN, LR [1 ]
ALTMAN, IB [1 ]
GUROV, MV [1 ]
KURKIN, AN [1 ]
机构：
[1] BIOTEKHNOL JOINT STOCK CO,INST BIOTECHNOL,DEPT MOLEC BIOL,MOSCOW,RUSSIA
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 1995年 / 119卷 / 03期
关键词：
INTERLEUKIN-10; GENE SYNTHESIS; PRODUCER STRAIN;
D O I：
10.1007/BF02445847
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The human interleukin-10 gene has been synthesized by the chemical enzymatic method. Vectors for cytoplasmic and periplasmic expression of recombinant interleukin-10 have been obtained in E. coli cells. A high level of protein expression was found to be characteristic of only recombinant strains producing interleukin-10 as ''fused'' protein (fused with the N-terminal fragment of interleukin-3).
引用
收藏
页码：316 / 319
页数：4
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