MOLECULAR CHARACTERIZATION OF THE ADP/ATP-TRANSPORTER CDNA FROM THE HUMAN MALARIA PARASITE PLASMODIUM-FALCIPARUM

We have isolated a cDNA sequence encoding the ADP/ATP transporter in Plasmodium falciparum. The sequence analysis revealed an open reading frame encoding 301 amino acids and showed significant similarities to known eukaryotic translocases such as that of Chlorella (up to 67.2% identity) acid the human transporter (61.2%). RNA blot analysis showed the presence of mRNA encoding for a 33.7-kDa ADP/ATP transporter. During the cell cycle of the parasite the expression levels of the transcripts fluctuate. The mitochondrial ADP/ATP transporter could play a role in energy metabolism of P. falciparum and makes this transporter an excellent target for chemotherapy.