DIRECT DETECTION OF YEAST MUTANTS WITH REDUCED VIABILITY ON PLATES BY ERYTHROSINE-B STAINING

被引：35

作者：

BONNEU, M ^{[1
]}

CROUZET, M ^{[1
]}

URDACI, M ^{[1
]}

AIGLE, M ^{[1
]}

机构：

[1] UNIV BORDEAUX 2,GENET LAB,ALLEE FAC,F-33405 TALENCE,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 193卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90013-J

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper we report a rapid method to screen yeast mutants exhibiting reduced viability directly on plates. This method avoids the need for replica plating and is based on the addition of the vital dye erythrosine B in nutrient medium. After 2 or 3 days of culture, colonies containing a large proportion of dead cells show a pink or a dark pink color whereas normal colonies are practically white. © 1991.

引用

页码：225 / 230

页数：6

共 17 条

[1]

BARONI MD, 1988, 14TH INT C YEAST GEN

[2]

COCCUCCI M, 1986, PLANT CELL ENVIRON, V9, P677

[3] INHIBITION OF PLASMA-MEMBRANE AND TONOPLAST ATPASES BY ERYTHROSIN-B [J].

COCUCCI, MC .

PLANT SCIENCE, 1986, 47 (01) :21-27

[4] VIABILITY MEASUREMENTS IN MAMMALIAN-CELL SYSTEMS [J].

COOK, JA ;

MITCHELL, JB .

ANALYTICAL BIOCHEMISTRY, 1989, 179 (01) :1-7

[5]

CULBERTSON MR, 1975, GENETICS, V80, P23

[6] BASIS OF DETERMINATION OF CELL VIABILITY WITH FLUOROCHROME PRIMULINE [J].

GRAHAM, RK .

JOURNAL OF THE INSTITUTE OF BREWING, 1970, 76 (01) :16-&

[7]

Matsumoto K, 1985, Yeast, V1, P15, DOI 10.1002/yea.320010103

[8]

PHILLIPS JH, 1973, TISSUE CULTURE METHO, P406

[9]

Pierce J. S., 1970, Journal of the Institute of Brewing, V76, P442

[10]

Rose A H, 1975, Methods Cell Biol, V12, P1

← 1 2 →