IMMUNOGLOBULIN HEAVY-CHAIN JOINING GENES IN THE RAT - COMPARISON WITH MOUSE AND HUMAN

被引：11
|
作者：
LANG, P [1 ]
MOCIKAT, R [1 ]
机构：
[1] GSF MUNICH,INST IMMUNOL,MARCHIONINISTR 25,W-8000 MUNICH 70,GERMANY
来源：
GENE | 1991年 / 102卷 / 02期
关键词：
RECOMBINANT DNA; J-GENE SEGMENTS; PSEUDOGENE; NUCLEOTIDE SEQUENCE; LOUVAIN RAT; POLYMERASE CHAIN REACTION;
D O I：
10.1016/0378-1119(91)90087-R
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have cloned by the polymerase chain reaction a 2.1-kb fragment carrying heavy-chain joining (J(H)) gene segments and a part of the J(H)-C-mu intron of the rat. Sequencing showed that the rat genome has four functional J(H) segments and that only a slight divergence has occurred after the separation of rat and mouse. A systematic sequence comparison between the two species and human revealed an additional J(H) pseudogene in rat and mouse 5' of J(H)1 which has not been described so far. The implications in evolutionary terms are discussed. In addition, we give an assessment of the misincorporation rate of the Taq polymerase.
引用
收藏
页码：261 / 264
页数：4
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