INDUCTION OF NITRITE PRODUCTION IN MOUSE SPLEEN-CELLS BY IMMUNIZATION

被引：9
作者：
TAKAGI, K
NAKAGAMI, H
NAKANO, T
SUKETA, Y
机构：
[1] Department of Environment Biochemistry, School of Pharmaceutical Science, University of Shizuoka, Shizuoka
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1092卷 / 01期
关键词：
NITRITE; INTERFERON-GAMMA; IMMUNE RESPONSE; T-CELL REGULATION;
D O I：
10.1016/0167-4889(91)90172-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Changes of nitrite production in mouse spleen cells of in vitro secondary antibody response were investigated. Mouse spleen cells immunized with gamma globulin fraction of rat serum produced nitrite 3 days after in vitro challenging with the same antigen. Nitrite production of rabbit IgG-challenged spleen cells was found to about 2.9-times higher than that of spleen cells primed with the gamma globulin fraction of rat serum. Nitrite production in this system was completely suppressed by T cell depletion (99.7% inhibition). Furthermore, nitrite production in these cells significantly decreased by addition of anti-interferon gamma antibody (62.9% inhibition). These data indicate that nitrite production in antigen-immunized spleen cells is affected with the immunogenicity of an antigen and regulated by T cells, especially interferon (IFN) gamma.
引用
收藏
页码：15 / 20
页数：6
相关论文
共 18 条
[1]   TIMING, GENETIC REQUIREMENTS AND FUNCTIONAL CONSEQUENCES OF SOMATIC HYPERMUTATION DURING B-CELL DEVELOPMENT [J].
ALLEN, D ;
CUMANO, A ;
DILDROP, R ;
KOCKS, C ;
RAJEWSKY, K ;
RAJEWSKY, N ;
ROES, J ;
SABLITZKY, F ;
SIEKEVITZ, M .
IMMUNOLOGICAL REVIEWS, 1987, 96 :5-22
[2]   MUTATION DRIFT AND REPERTOIRE SHIFT IN THE MATURATION OF THE IMMUNE-RESPONSE [J].
BEREK, C ;
MILSTEIN, C .
IMMUNOLOGICAL REVIEWS, 1987, 96 :23-41
[3]  
BRADLEY LM, 1980, SELECTED METHODS CEL, P156
[4]  
DING AH, 1988, J IMMUNOL, V141, P2407
[5]   THE ROLE OF SOMATIC HYPERMUTATION IN THE GENERATION OF ANTIBODY DIVERSITY [J].
FRENCH, DL ;
LASKOV, R ;
SCHARFF, MD .
SCIENCE, 1989, 244 (4909) :1152-1157
[6]   ANALYSIS OF NITRATE, NITRITE, AND [N-15]-LABELED NITRATE IN BIOLOGICAL-FLUIDS [J].
GREEN, LC ;
WAGNER, DA ;
GLOGOWSKI, J ;
SKIPPER, PL ;
WISHNOK, JS ;
TANNENBAUM, SR .
ANALYTICAL BIOCHEMISTRY, 1982, 126 (01) :131-138
[7]   NITRIC-OXIDE - A CYTO-TOXIC ACTIVATED MACROPHAGE EFFECTOR MOLECULE [J].
HIBBS, JB ;
TAINTOR, RR ;
VAVRIN, Z ;
RACHLIN, EM .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 157 (01) :87-94
[8]   INVITRO CHARACTERIZATION OF THE FIBROIN GENE PROMOTER BY THE USE OF SINGLE-BASE SUBSTITUTION MUTANTS [J].
HIROSE, S ;
TAKEUCHI, K ;
SUZUKI, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (23) :7258-7262
[9]   ENDOTHELIUM-DERIVED NITRIC-OXIDE - ACTIONS AND PROPERTIES [J].
IGNARRO, LJ .
FASEB JOURNAL, 1989, 3 (01) :31-36
[10]   HIGH-RATE OF SOMATIC POINT MUTATION INVITRO IN AND NEAR THE VARIABLE-REGION SEGMENT OF AN IMMUNOGLOBULIN HEAVY-CHAIN GENE [J].
MEYER, J ;
JACK, HM ;
ELLIS, N ;
WABL, M .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (18) :6950-6953
12