COMPARISON OF NONSPECIFIC RADIOIMMUNOASSAY, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, AND FLUORESCENCE POLARIZATION IMMUNOASSAY FOR CYCLOSPORINE MONITORING IN RENAL-TRANSPLANTATION

被引：8
|
作者：
LEE, SF
YANG, WC
SHANN, TY
LUI, WY
WANG, RB
机构：
[1] VET GEN HOSP TAIPEI,DIV NEPHROL,TAIPEI 11217,TAIWAN
[2] VET GEN HOSP TAIPEI,DEPT GEN SURG,TAIPEI 11217,TAIWAN
来源：
THERAPEUTIC DRUG MONITORING | 1991年 / 13卷 / 02期
关键词：
CYCLOSPORINE; RADIOIMMUNOASSAY; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; FLUORESCENCE POLARIZATION IMMUNOASSAY;
D O I：
10.1097/00007691-199103000-00011
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Three methods, i.e., nonspecific radioimmunoassay (RIA; Incstar), fluorescence polarization immunoassay (FPIA; TDx Abbott), and high-performance liquid chromatography (HPLC), have been used for monitoring cyclosporine blood levels in renal transplantation patients. The levels obtained from 135 samples showed a modest correlation between RIA and HPLC, FPIA and HPLC, RIA and FRIA. The mean ratios of RIA to HPLC, FPIA to HPLC, and RIA to FPIA were 2.96, 4.14, and 0.73. The significant variations in cyclosporine levels result from the cross-reaction of antibody with some cyclosporine metabolites, by which these two methods often overestimate the true blood cyclosporine level. HPLC is a more effective and reliable method for pharmacokinetic studies and blood level monitoring of cyclosporine in clinical practice.
引用
收藏
页码：152 / 156
页数：5
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