A METHOD FOR THE PREPARATION OF COIMMOBILIZATES BY ADHESION USING POLYETHYLENIMINE

被引：17
|
作者：
DSOUZA, SF
MELO, JS
机构：
[1] Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 06期
关键词：
COIMMOBILIZATE; POLYETHYLENIMINE; YEAST CELLS; GLUCOSE OXIDASE; COTTON THREAD; ADHESION;
D O I：
10.1016/0141-0229(91)90011-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method has been described for obtaining coimmobilizates by the simultaneous binding of glucose oxidase to the cell and the enzyme-bound cell to cotton thread through adhesion using polyethylenimine (PEI). Glucose oxidase was found to adsorb onto PEI-coated yeast cells from a water suspension. The desorption observed at higher ionic strength could be obviated by cross-linking with 2% glutaraldehyde for 2 min. The enzyme-bound yeast cells could then be immobilized by adhesion on cotton thread. The coimmobilizate could be reused for over 10 batches without appreciable loss in activity.
引用
收藏
页码：508 / 511
页数：4
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