A METHOD FOR THE PREPARATION OF COIMMOBILIZATES BY ADHESION USING POLYETHYLENIMINE

被引：17
作者：
DSOUZA, SF
MELO, JS
机构：
[1] Food Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1991年 / 13卷 / 06期
关键词：
COIMMOBILIZATE; POLYETHYLENIMINE; YEAST CELLS; GLUCOSE OXIDASE; COTTON THREAD; ADHESION;
D O I：
10.1016/0141-0229(91)90011-X
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method has been described for obtaining coimmobilizates by the simultaneous binding of glucose oxidase to the cell and the enzyme-bound cell to cotton thread through adhesion using polyethylenimine (PEI). Glucose oxidase was found to adsorb onto PEI-coated yeast cells from a water suspension. The desorption observed at higher ionic strength could be obviated by cross-linking with 2% glutaraldehyde for 2 min. The enzyme-bound yeast cells could then be immobilized by adhesion on cotton thread. The coimmobilizate could be reused for over 10 batches without appreciable loss in activity.
引用
收藏
页码：508 / 511
页数：4
相关论文
共 22 条
[1]   IMMOBILIZATION OF ACETOBACTER-ACETI ON CELLULOSE ION-EXCHANGERS - ADSORPTION-ISOTHERMS [J].
BAR, R ;
GAINER, JL ;
KIRWAN, DJ .
BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (08) :1166-1171
[2]   ENHANCED YEAST IMMOBILIZATION BY NUTRIENT STARVATION [J].
BRINGI, V ;
DALE, BE .
BIOTECHNOLOGY LETTERS, 1985, 7 (12) :905-908
[3]   CONTINUOUS CONVERSION OF SUCROSE TO FRUCTOSE AND GLUCONIC ACID BY IMMOBILIZED YEAST-CELL MULTI-ENZYME COMPLEX [J].
DSOUZA, SF ;
NADKARNI, GB .
BIOTECHNOLOGY AND BIOENGINEERING, 1980, 22 (10) :2179-2189
[4]   A METHOD FOR THE PREPARATION OF HEN EGG-WHITE BEADS CONTAINING IMMOBILIZED BIOCATALYSTS [J].
DSOUZA, SF ;
KAUL, R ;
NADKARNI, GB .
BIOTECHNOLOGY LETTERS, 1985, 7 (08) :589-592
[5]  
DSOUZA SF, 1988, APPL MICROBIOL BIOT, V29, P136
[6]   IMMOBILIZATION OF YEAST-CELLS BY ADHESION TO GLASS-SURFACE USING POLYETHYLENIMINE [J].
DSOUZA, SF ;
MELO, JS ;
DESHPANDE, A ;
NADKARNI, GB .
BIOTECHNOLOGY LETTERS, 1986, 8 (09) :643-648
[7]   CONTINUOUS INVERSION OF SUCROSE BY GEL-ENTRAPPED YEAST-CELLS [J].
DSOUZA, SF ;
NADKARNI, GB .
ENZYME AND MICROBIAL TECHNOLOGY, 1980, 2 (03) :217-222
[8]  
HAGERDAL B, 1980, FOOD PROCESS ENG, V2, P129
[9]  
HARTMEIER W, 1984, PROCESS BIOCHEM, V19, P40
[10]  
HARTMEIER W, 1983, ENZYME TECHNOLOGY, P207
123