DETERMINATION OF C-N INTERNUCLEAR DISTANCES BY ROTATIONAL-ECHO DOUBLE-RESONANCE NMR OF SOLIDS

被引：186

作者：

PAN, Y

GULLION, T

SCHAEFER, J

机构：

[1] Department of Chemistry, Washington University, St. Louis

来源：

JOURNAL OF MAGNETIC RESONANCE | 1990年 / 90卷 / 02期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1016/0022-2364(90)90138-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Rotational-echo, double-resonance (REDOR) 15N13C NMR has been performed on an alanine cocrystallized from five-component alanines, isotopically enriched in 13C, 15N, or 12C. REDOR 15N13C NMR involves the dephasing of carbon magnetization by 15N 180° pulses synchronized with magic-angle spinning. The CN dipolar coupling determines the extent of dephasing. The results of these experiments on alanine show that it is practical to use REDOR to measure the CN dipolar coupling of 5 μmol of a 13C15N-labeled pair having an Internuclear separation of the order of 4.5 Å. © 1990.

引用

收藏

页码：330 / 340

页数：11

相关论文

共 13 条

[1] THE CRYSTAL STRUCTURE OF DL-ALANINE .2. REVISION OF PARAMETERS BY 3-DIMENSIONAL FOURIER ANALYSIS [J].

DONOHUE, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1950, 72 (02) :949-953

[2] ROTATIONAL-ECHO DOUBLE-RESONANCE NMR [J].

GULLION, T ;

SCHAEFER, J .

JOURNAL OF MAGNETIC RESONANCE, 1989, 81 (01) :196-200

[3]

GULLION T, 1989, ADV MAGN RESON, V13, P57

[4] COHERENT AVERAGING EFFECTS IN MAGNETIC RESONANCE [J].

HAEBERLEN, U ;

WAUGH, JS .

PHYSICAL REVIEW, 1968, 175 (02) :453-+

[5] PRECISION NEUTRON-DIFFRACTION STRUCTURE DETERMINATION OF PROTEIN AND NUCLEIC-ACID COMPONENTS .1. CRYSTAL AND MOLECULAR STRUCTURE OF AMINO-ACID L-ALANINE [J].

LEHMANN, MS ;

KOETZLE, TF ;

HAMILTON, WC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1972, 94 (08) :2657-&

[6] FREE INDUCTION DECAYS OF ROTATING SOLIDS [J].

LOWE, IJ .

PHYSICAL REVIEW LETTERS, 1959, 2 (07) :285-287

[7] DETERMINATION OF A PRECISE INTERATOMIC DISTANCE IN A HELICAL PEPTIDE BY REDOR NMR [J].

MARSHALL, GR ;

BEUSEN, DD ;

KOCIOLEK, K ;

REDLINSKI, AS ;

LEPLAWY, MT ;

PAN, Y ;

SCHAEFER, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (03) :963-966

[8] TWO-DIMENSIONAL NUCLEAR MAGNETIC-RESONANCE IN ROTATING SOLIDS - AN ANALYSIS OF LINE-SHAPES IN CHEMICAL SHIFT-DIPOLAR SPECTRA [J].

MUNOWITZ, MG ;

GRIFFIN, RG .

JOURNAL OF CHEMICAL PHYSICS, 1982, 76 (06) :2848-2858

[9] DANTE-SELECTED ROTATIONAL-ECHO DOUBLE-RESONANCE NMR [J].

PAN, Y ;

SCHAEFER, J .

JOURNAL OF MAGNETIC RESONANCE, 1990, 90 (02) :341-345

[10] DIPOLAR ROTATIONAL SPIN-ECHO C-13 NMR OF POLYMERS [J].

SCHAEFER, J ;

MCKAY, RA ;

STEJSKAL, EO .

JOURNAL OF MAGNETIC RESONANCE, 1983, 52 (01) :123-129