VASOACTIVE-INTESTINAL-PEPTIDE RECEPTORS ON AR42J RAT PANCREATIC ACINAR-CELLS

被引：13
作者：
RAYMOND, MJ
ROSENZWEIG, SA
机构：
[1] YALE UNIV,SCH MED,DEPT OPHTHALMOL & VISUAL SCI,NEW HAVEN,CT 06510
[2] YALE UNIV,SCH MED,DEPT CELL BIOL,NEW HAVEN,CT 06510
[3] MED UNIV S CAROLINA,DEPT CELL & MOLEC PHARMACOL & EXPTL THERAPEUT,CHARLESTON,SC 29425
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 179卷 / 01期
关键词：
D O I：
10.1016/0006-291X(91)91351-C
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
VIP receptors on AR42J rat pancreatic cells were analyzed by competition binding, affinity labeling and by N-glycanase digestion analyses. These studies revealed the presence of specific, high affinity (Kd ∼ 1 nM) VIP receptors with a mass of 68 kDa or 59 kDa under reducing or non-reducing conditions, respectively. N-glycanase digestion of affinity labeled membranes generated a core receptor protein of ∼44 kDa and evidence for at least two N-linked glycans on the mature receptor. The receptor lacked O-linked oligosaccharides but contained terminal sialic acid residues of its N-linked glycan(s) based on digestions with O-glycanase and neuraminidase. The similarity of the AR42J VIP receptor to the recently cloned cDNA for human VIP receptors makes this cell line an attractive model for further analysis of VIP receptor signal transduction events. © 1991.
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页码：176 / 182
页数：7
相关论文
共 28 条
[1]   RECEPTORS FOR VASOACTIVE INTESTINAL PEPTIDE AND SECRETIN ON RAT PANCREATIC ACINI [J].
BISSONNETTE, BM ;
COLLEN, MJ ;
ADACHI, H ;
JENSEN, RT ;
GARDNER, JD .
AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 246 (06) :G710-G717
[2]  
BROWN PJ, 1990, J BIOL CHEM, V29, P17995
[3]   THE RAT-LIVER VASOACTIVE INTESTINAL PEPTIDE BINDING-SITE - MOLECULAR CHARACTERIZATION BY COVALENT CROSS-LINKING AND EVIDENCE FOR DIFFERENCES FROM THE INTESTINAL RECEPTOR [J].
COUVINEAU, A ;
LABURTHE, M .
BIOCHEMICAL JOURNAL, 1985, 225 (02) :473-479
[4]   VASOACTIVE INTESTINAL POLYPEPTIDE - MEASUREMENT, DISTRIBUTION AND PUTATIVE NEUROTRANSMITTER FUNCTION [J].
FAHRENKRUG, J .
DIGESTION, 1979, 19 (03) :149-169
[5]  
INOUE Y, 1985, ENDOCRINOLOGY, V11, P686
[6]  
JESSOP NW, 1980, IN VITRO CELL DEV B, V16, P212
[7]   MOLECULAR-IDENTIFICATION OF RECEPTORS FOR VASOACTIVE INTESTINAL PEPTIDE IN RAT INTESTINAL EPITHELIUM BY COVALENT CROSS-LINKING - EVIDENCE FOR 2 CLASSES OF BINDING-SITES WITH DIFFERENT STRUCTURAL AND FUNCTIONAL-PROPERTIES [J].
LABURTHE, M ;
BREANT, B ;
ROUYERFESSARD, C .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 139 (01) :181-187
[8]   CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].
LAEMMLI, UK .
NATURE, 1970, 227 (5259) :680-+
[9]   GLUCOCORTICOIDS INCREASE AMYLASE MESSENGER-RNA LEVELS, SECRETORY ORGANELLES, AND SECRETION IN PANCREATIC ACINAR AR42J CELLS [J].
LOGSDON, CD ;
MOESSNER, J ;
WILLIAMS, JA ;
GOLDFINE, ID .
JOURNAL OF CELL BIOLOGY, 1985, 100 (04) :1200-1208
[10]  
LOGSDON CD, 1986, J BIOL CHEM, V261, P2096
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