N-CADHERIN GENE MAPS TO HUMAN CHROMOSOME-18 AND IS NOT LINKED TO THE E-CADHERIN GENE

被引：86

作者：

WALSH, FS ^{[1
]}

BARTON, CH ^{[1
]}

PUTT, W ^{[1
]}

MOORE, SE ^{[1
]}

KELSELL, D ^{[1
]}

SPURR, N ^{[1
]}

GOODFELLOW, PN ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,LONDON WC2A 3PX,ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1990年 / 55卷 / 03期

基金：

英国惠康基金;

关键词：

Cell adhesion molecules; Gene mapping; N‐Cadherin;

D O I：

10.1111/j.1471-4159.1990.tb04563.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Abstract: cDNA clones encoding the human N‐cadherin cell adhesion molecule have been isolated from an embryonic muscle library by screening with an oligonucleotide probe complementary to the chick brain sequence and chick brain cDNA probe λN2. Comparison of the predicted protein sequences revealed >91% homology between chick brain, mouse brain, and human muscle N‐cadherin cDNAs over the 748 amino acids of the mature, processed protein. A single polyadenylation site in the chick clone was also present and duplicated in the human muscle sequence. Immediately 3’of the recognition site in chick a poly(A) tail ensued; however, in human an additional 800 bp of 3’untranslated sequence followed. Northern analysis identified a number of major N‐cadherin mRNAs. These were of 5.2, 4.3, and 4.0 kb in C6 glioma, 4.3 and 4.0 kb in human foetal muscle cultures, and 4.3 kb in human embryonic brain and mouse brain with minor bands of 5.2 kb in human muscle and embryonic brain. Southern analysis of a panel of somatic cell hybrids allowed the human N‐cadherin gene to be mapped to chromosome 18. This is distinct from the E‐cadherin locus on chromosome 16. Therefore, it is likely that the cadherins have evolved from a common precursor gene that has undergone duplication and migration to other chromosomal locations. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：805 / 812

页数：8

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