STUDIES OF GYNOGENESIS IN ONION (ALLIUM-CEPA L) - INDUCTION PROCEDURES AND GENETIC-ANALYSIS OF REGENERANTS

被引：61

作者：

BOHANEC, B ^{[1
]}

JAKSE, M ^{[1
]}

IHAN, A ^{[1
]}

JAVORNIK, B ^{[1
]}

机构：

[1] UNIV LJUBLJANA,FAC MED,INST MICROBIOL,LJUBLJANA 61000,SLOVENIA

来源：

PLANT SCIENCE | 1995年 / 104卷 / 02期

关键词：

ALLIUM CEPA; ONION; HAPLOID PLANTS; GYNOGENESIS; THIDIAZURON; FLOW CYTOMETRY; ISOZYMES; RAPD; GAMETOCLONAL VARIATION;

D O I：

10.1016/0168-9452(94)04030-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gynogenesis of four onion cultivars was induced, starting from ovules and ovaries, using a 2-step culture procedure. The induction frequency was much higher from ovaries and was strongly affected by genotype. Thidiazuron (2 mg/l) had a positive effect on regeneration and subsequent in vitro culture of regenerants. Esterase isozyme analysis showed that 59% of regenerants were homozygous. Chromosome counting of root tip cells and flow cytometry of leaf tissue showed haploid, doubled haploid and mixoploid structures. Within cloned plants, some showed segregation of the ploidy level with a tendency towards endoreduplication. Analysis of random amplified polymorphic DNA (RAPD) profiles of homozygous regenerants revealed several missing bands compared with donor plants, but micropropagated clones of different ploidy level were identical among each other. Gametoclonal variation was detected by the appearance of a novel RAPD band, present in two out of 12 regenerants and not in the donor plants.

引用

页码：215 / 224

页数：10

共 50 条

[31] Genetic variation of quercetin glucoside content in onion (Allium cepa L.)
Okamoto, D
Noguchi, Y
Muro, T
Morishita, M
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 2006, 75 (01): : 100 - 108
[32] Genetic Divergence and Its Importance for Breeding Onion (Allium cepa L.)
Pavlovic, N.
Cvikic, D.
Zdravkovic, J.
Mijatovic, M.
IV BALKAN SYMPOSIUM ON VEGETABLES AND POTATOES, 2009, 830 : 235 - 239
[33] Dynamic management of genetic resources of Allium cepa L (onion) in west Africa
Rouamba, A
Ricroch, A
Sarr, A
FIRST INTERNATIONAL SYMPOSIUM ON EDIBLE ALLIACEAE, 1997, (433): : 185 - 189
[34] INFLUENCE OF COLD PRETREATMENTS ON CALLUS AND EMBRYO INDUCTION IN ONION (ALLIUM CEPA L.) VIA FLOWER BUD CULTURE
Karakan, Faika Yarali
FRESENIUS ENVIRONMENTAL BULLETIN, 2019, 28 (11): : 8070 - 8075
[35] Allium cepa L (onion) .2. Chemistry, Analysis and Pharmacology
Dorsch, W
PHYTOMEDICINE, 1997, 3 (04) : 391 - 397
[36] The genetic relatedness analysis of male sterile and their maintainer lines of onion (Allium cepa L.) by using RAPD primers
Dhanya Shivnanajappa
Lakshmana Reddy Dhoranalapalli Chinnappareddy
Verre Gowda
Sreedhara Sindughatta Antharamiah
Aswath Chennareddy
Journal of Crop Science and Biotechnology, 2013, 16 (1) : 29 - 33
[37] Genetic Relatedness Between Quantitative and Qualitative Parameters in Onion (Allium cepa L.)
Chattopadhyay, Arup
Sharangi, Amit Baran
Dutta, Saheb
Das, Sibsankar
Denre, Manas
VEGETOS, 2013, 26 (01): : 151 - 157
[38] Cultivar identification and genetic diversity in onion (Allium cepa L.) as evaluated by random amplified polymorphic DNA (RAPD) analysis
Tanikawa, T
Takagi, M
Ichii, M
JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE, 2002, 71 (02): : 249 - 251
[39] ANTIMICROBIAL EFFECTS OF ALLIUM-SATIVUM L (GARLIC), ALLIUM-AMPELOPRASUM L (ELEPHANT GARLIC), AND ALLIUM-CEPA L (ONION), GARLIC COMPOUNDS AND COMMERCIAL GARLIC SUPPLEMENT PRODUCTS
HUGHES, BG
LAWSON, LD
PHYTOTHERAPY RESEARCH, 1991, 5 (04) : 154 - 158
[40] Identification of variables for can be used as approaches of selection in programs of genetic improvement of the onion (Allium cepa L.)
Lescay Batista, Elio
Gonzalez Nunez, Luis M.
CENTRO AGRICOLA, 2011, 38 (03): : 23 - 28

← 1 2 3 4 5 →