STUDIES OF GYNOGENESIS IN ONION (ALLIUM-CEPA L) - INDUCTION PROCEDURES AND GENETIC-ANALYSIS OF REGENERANTS

被引：64

作者：

BOHANEC, B ^{[1
]}

JAKSE, M ^{[1
]}

IHAN, A ^{[1
]}

JAVORNIK, B ^{[1
]}

机构：

[1] UNIV LJUBLJANA,FAC MED,INST MICROBIOL,LJUBLJANA 61000,SLOVENIA

来源：

PLANT SCIENCE | 1995年 / 104卷 / 02期

关键词：

ALLIUM CEPA; ONION; HAPLOID PLANTS; GYNOGENESIS; THIDIAZURON; FLOW CYTOMETRY; ISOZYMES; RAPD; GAMETOCLONAL VARIATION;

D O I：

10.1016/0168-9452(94)04030-K

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gynogenesis of four onion cultivars was induced, starting from ovules and ovaries, using a 2-step culture procedure. The induction frequency was much higher from ovaries and was strongly affected by genotype. Thidiazuron (2 mg/l) had a positive effect on regeneration and subsequent in vitro culture of regenerants. Esterase isozyme analysis showed that 59% of regenerants were homozygous. Chromosome counting of root tip cells and flow cytometry of leaf tissue showed haploid, doubled haploid and mixoploid structures. Within cloned plants, some showed segregation of the ploidy level with a tendency towards endoreduplication. Analysis of random amplified polymorphic DNA (RAPD) profiles of homozygous regenerants revealed several missing bands compared with donor plants, but micropropagated clones of different ploidy level were identical among each other. Gametoclonal variation was detected by the appearance of a novel RAPD band, present in two out of 12 regenerants and not in the donor plants.

引用

页码：215 / 224

页数：10

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