RECOVERY OF ISOAMYLASE FROM PSEUDOMONAS-AMYLODERAMOSA BY ADSORPTION-ELUTION ON RAW STARCH

被引：24
|
作者：
FANG, TY [1 ]
LIN, LL [1 ]
HSU, WH [1 ]
机构：
[1] FOOD IND RES & DEV INST, CULTURE COLLECT & RES CTR, HSINCHU, TAIWAN
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1994年 / 16卷 / 03期
关键词：
PSEUDOMONAS AMYLODERAMOSA; ISOAMYLASE; RAW STARCH ADSORPTION;
D O I：
10.1016/0141-0229(94)90050-7
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The isoamylase from Pseudomonas amyloderamosa WU2130 was adsorbed onto raw starch, and the adsorbed enzyme was eluted by maltose in 50 mM acetate buffer. The adsorption of isoamylase to adsorbent was affected by the sour ces of raw starch, temperature, and pH, whereas the temperature and elution time had no obvious effects on the elution of adsorbed isoamylase. Raw starch could be used repeatedly on the adsorption-elution cycle,vith good reproducibility. About 52.5% of isoamylase was recovered, and the eluted enzyme had a specific activity of 72,828 U mg(-1) protein with a purification of 13.3-fold. These results suggest that the law starch adsorption-elution technique has a great potential in recovery of isoamylase from culture broths.
引用
收藏
页码：247 / 252
页数：6
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