REVERSE-PHASE LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM MASS-SPECTROMETRY IN THE ANALYSIS OF PEPTIDES

被引：20

作者：

STACEY, CC ^{[1
]}

KRUPPA, GH ^{[1
]}

WATSON, CH ^{[1
]}

WRONKA, J ^{[1
]}

LAUKIEN, FH ^{[1
]}

BANKS, JF ^{[1
]}

WHITEHOUSE, CM ^{[1
]}

机构：

[1] ANALYT BRANFORD,BRANFORD,CT 06405

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 07期

关键词：

D O I：

10.1002/rcm.1290080704

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Electrospray ionization (ESI) in combination with Fourier-transform mass spectrometry (FTMS) gives both highly accurate mass analysis of peptides with external calibration and high resolution for charge-state determination of an ion from the naturally occurring isotopic peaks. Liquid chromatographic (LC) separation of peptides is important for the analysis of complex mixtures. High-resolution, high accuracy measurement of the molecular ions of the mix of peptides has been achieved with LC/ESI-FTMS. Examples are given to show both the reproducibility of mass accuracy over replicate analyses and to show the utility of broadband analysis of a gradient separation of peptides. The high mass accuracy and resolution of FTMS was uncompromised for the direct analysis of chromatography peaks.

引用

页码：513 / 516

页数：4

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