REVERSE-PHASE LIQUID-CHROMATOGRAPHY ELECTROSPRAY-IONIZATION FOURIER-TRANSFORM MASS-SPECTROMETRY IN THE ANALYSIS OF PEPTIDES

被引：20
|
作者：
STACEY, CC [1 ]
KRUPPA, GH [1 ]
WATSON, CH [1 ]
WRONKA, J [1 ]
LAUKIEN, FH [1 ]
BANKS, JF [1 ]
WHITEHOUSE, CM [1 ]
机构：
[1] ANALYT BRANFORD,BRANFORD,CT 06405
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1994年 / 8卷 / 07期
关键词：
D O I：
10.1002/rcm.1290080704
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Electrospray ionization (ESI) in combination with Fourier-transform mass spectrometry (FTMS) gives both highly accurate mass analysis of peptides with external calibration and high resolution for charge-state determination of an ion from the naturally occurring isotopic peaks. Liquid chromatographic (LC) separation of peptides is important for the analysis of complex mixtures. High-resolution, high accuracy measurement of the molecular ions of the mix of peptides has been achieved with LC/ESI-FTMS. Examples are given to show both the reproducibility of mass accuracy over replicate analyses and to show the utility of broadband analysis of a gradient separation of peptides. The high mass accuracy and resolution of FTMS was uncompromised for the direct analysis of chromatography peaks.
引用
收藏
页码：513 / 516
页数：4
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