A CONVENIENT CLONING VECTOR CONTAINING THE GAL4 DNA-BINDING DOMAIN

被引：3
|
作者：
RAYCROFT, L [1 ]
LOZANO, G [1 ]
机构：
[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,1515 HOLCOMBE BLVD,BOX 11,HOUSTON,TX 77030
来源：
GENE | 1992年 / 118卷 / 01期
关键词：
RECOMBINANT DNA; FUSION PROTEINS; TRANSACTIVATION;
D O I：
10.1016/0378-1119(92)90262-N
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation of fusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.
引用
收藏
页码：143 / 144
页数：2
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