CLONING AND SEQUENCING THE UROCANASE GENE (HUTU) FROM PSEUDOMONAS-PUTIDA

被引：15

作者：

FESSENMAIER, M

FRANK, R

RETEY, J

SCHUBERT, C

机构：

[1] UNIV KARLSRUHE,INST ORGAN CHEM,LEHRSTUHL BIOCHEM,W-7500 KARLSRUHE 1,GERMANY

[2] UNIV HEIDELBERG,CTR MOLEC BIOL,W-6900 HEIDELBERG,GERMANY

来源：

FEBS LETTERS | 1991年 / 286卷 / 1-2期

关键词：

UROCANASE; HUTU GENE; PCR; PSEUDOMONAS-PUTIDA;

D O I：

10.1016/0014-5793(91)80938-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A clone harbouring the entire urocanase gene (hutU) was obtained from a genomic library of Pseudomonas putida using oligonucleotide probes synthesised on the basis of known flanking sequences. One subunit of urocanase consists of 556 amino acids and has a molecular mass of 60 771 Da.

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页码：55 / 57

页数：3

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