INCREASED LEVEL OF ATRIAL-NATRIURETIC-PEPTIDE MESSENGER-RNA IN THE HYPOTHALAMUS AND BRAIN-STEM OF SPONTANEOUSLY HYPERTENSIVE RATS

被引：15

作者：

KOMATSU, K ^{[1
]}

TANAKA, I ^{[1
]}

FUNAI, T ^{[1
]}

ICHIYAMA, A ^{[1
]}

YOSHIMI, T ^{[1
]}

机构：

[1] HAMAMATSU UNIV SCH MED, DEPT BIOCHEM 1, HAMAMATSU 43131, JAPAN

来源：

JOURNAL OF HYPERTENSION | 1992年 / 10卷 / 01期

关键词：

ATRIAL NATRIURETIC PEPTIDE; MESSENGER RNA; SPONTANEOUSLY HYPERTENSIVE RATS; BRAIN; RIBONUCLEASE PROTECTION ASSAY;

D O I：

10.1097/00004872-199201000-00004

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

Objective: The aim of this study was to investigate atrial natriuretic peptide (ANP) gene expression in the central nervous system (CNS) during hypertension. Methods: We measured and compared immunoreactive atrial natriuretic peptide (irANP) and ANP messenger RNA (mRNA) in the hypothalamus and brainstem of 17-week-old spontaneously hypertensive rats (SHR) with those of age-matched Wistar-Kyoto (WKY) rats using ribonuclease (RNase) protection assay for ANP mRNA and a specific radioimmunoassay for irANP. Results: RNase protection assay revealed that the concentrations of ANP mRNA in the hypothalamus and brainstem of SHR were higher than those of WKY rats. IrANP concentrations in the hypothalamus and brainstem of SHR were determined by a specific radioimmunoassay and found to be higher than those of WKY rats. Elevated mRNA levels in the hypothalamus and brainstem of SHR indicated that increased level of irANP in the CNS resulted from increased synthesis of ANP. Conclusion: We propose that increased synthesis of brain ANP in SHR may reflect a compensatory mechanism induced by hypertension.

引用

页码：17 / 23

页数：7

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