SECRETION OF A POTENT NEW MIGRATION FACTOR FOR SMOOTH-MUSCLE CELLS (SMC) BY CULTURED SMC

被引：24

作者：

KOYAMA, N ^{[1
]}

KOSHIKAWA, T ^{[1
]}

MORISAKI, N ^{[1
]}

SAITO, Y ^{[1
]}

YOSHIDA, S ^{[1
]}

机构：

[1] CHIBA UNIV,SCH MED,DEPT INTERNAL MED 2,1-8-1 INOHANA,CHIBA 280,JAPAN

来源：

ATHEROSCLEROSIS | 1991年 / 86卷 / 2-3期

关键词：

SMC MIGRATION; AUTOCRINE SYSTEM; CHEMOTACTIC FACTOR; INTIMAL THICKENING;

D O I：

10.1016/0021-9150(91)90218-R

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Migration of smooth muscle cells (SMC) in the arterial wall is important in the formation of intimal thickening. In this work, cultured SMC from the rat and rabbit aortic media at 2nd to 12th passages were found to secrete a potent migration factor for SMC which was named SMC-derived migration factor (SDMF). This factor stimulated the migration of SMC dose-dependently and its maximum activity was 2-8 times that of PDGF. Checker board analysis showed that SDMF was chemotactic, but not chemokinetic. In further studies, SDMF was found to be inactivated at 100-degrees-C for 10 min or by trypsinization, but not inactivated by mercaptoethanol. This factor was not dialyzable. Molecular weight was approximately 500 kDa by a gel filtration. The activity was not inhibited by an anti-PDGF antibody or a fibronectin antiserum. These data suggest that SDMF is a potent migration factor for SMC and that SDMF is distinct from PDGF, fibronectin or other known migration factors. This autocrine system of secretion of SDMF by SMC and its induction of SMC migration may contribute to intimal thickening of the arterial wall in atherosclerosis.

引用

页码：219 / 226

页数：8

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