CHARACTERIZATION AND REGULATION OF PHOSPHATIDYLGLYCEROLPHOSPHATE PHOSPHATASE IN SACCHAROMYCES-CEREVISIAE

被引：47
|
作者：
KELLY, BL
GREENBERG, ML
机构：
[1] UNIV MICHIGAN,SCH MED,DEPT BIOL CHEM,1301 CATHERINE RD,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,SCH MED,CELLULAR & MOLEC BIOL PROGRAM,ANN ARBOR,MI 48109
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1046卷 / 02期
关键词：
(S. cerevisiae); Enzyme regulation; Mitochondrial membrane; Phosphatidylglycerolphosphate phosphatase; Phospholipid synthesis;
D O I：
10.1016/0005-2760(90)90181-V
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Phosphatidylglycerophosphatase (EC 3.1.3.27) activity was characterized in mitochondrial extracts from Saccharomyces cerevisiae. The enzyme has a pH optimum of 5.5. Maximum activity occurs in the presence of Triton X-100 (5 mM) and cobalt or magnesium ions (5 mM). The apparent Km for PGP is 14.6 μM. The temperature optimum is between 50°C and 60°C. The enzyme is labile above 50°C. The presence of inositol in growth media results in a slight but reproducible increase in PGPase activity in mitochondrial extracts from glucose-grown cells but not glycerol-grown cells. The inositol effect is not seen in crude cell extracts. Carbon source does not affect PGPase activity in mitochondrial extracts or in crude cell extracts. © 1990.
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收藏
页码：144 / 150
页数：7
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