SCANNING SLIT APERTURE CONFOCAL MICROSCOPY FOR 3-DIMENSIONAL IMAGING

被引：6
作者：
BURNS, DH
HATANGADI, RB
SPELMAN, FA
机构：
[1] Center for Bioengineering, University of Washington, Seattle, Washington
来源：
SCANNING | 1990年 / 12卷 / 03期
关键词：
D O I：
10.1002/sca.4950120307
中图分类号：
TH7 [仪器、仪表];
学科分类号：
0804 ; 080401 ; 081102 ;
摘要：
A scanning confocal microscope using stationary slit apertures of variable width is described. Scanning is achieved by employing an oscillating mirror galvanometer capable of scanning images at 160 frames/s. The microscope depth‐of‐focus is characterized for several slit widths and shown to approach diffraction‐limited performance as width decreases. Reduction of flare in images taken with narrow slit widths is demonstrated. In addition, the optical sectioning capability of the system is demonstrated using polymer casts of the vasculature of rat kidney. Copyright © 1990 Foundation for Advances in Medicine and Science, Inc.
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页码：156 / 160
页数：5
相关论文
共 13 条
  • [1] BOYDE A, 1986, ANN NY ACAD SCI, V483, P405
  • [2] CONFOCAL SCANNING LIGHT-MICROSCOPY WITH HIGH APERTURE IMMERSION LENSES
    BRAKENHOFF, GJ
    BLOM, P
    BARENDS, P
    [J]. JOURNAL OF MICROSCOPY-OXFORD, 1979, 117 (NOV): : 219 - 232
  • [3] 3-DIMENSIONAL MICROSCOPY USING A CONFOCAL LASER SCANNING MICROSCOPE
    CARLSSON, K
    DANIELSSON, PE
    LENZ, R
    LILJEBORG, A
    MAJLOF, L
    ASLUND, N
    [J]. OPTICS LETTERS, 1985, 10 (02) : 53 - 55
  • [4] DEPTH RESPONSE OF CONFOCAL OPTICAL MICROSCOPES
    CORLE, TR
    CHOU, CH
    KINO, GS
    [J]. OPTICS LETTERS, 1986, 11 (12) : 770 - 772
  • [5] OBSERVATION OF NERVE FIBERS IN INCIDENT LIGHT
    EGGER, MD
    GEZARI, W
    DAVIDOVITS, P
    HADRAVSKY, M
    PETRAN, M
    [J]. EXPERIENTIA, 1969, 25 (11): : 1225 - +
  • [6] NEW REFLECTED-LIGHT MICROSCOPE FOR VIEWING UNSTAINED BRAIN AND GANGLION CELLS
    EGGER, MD
    PETRAN, M
    [J]. SCIENCE, 1967, 157 (3786) : 305 - &
  • [7] EXPERIMENTAL-OBSERVATIONS OF THE DEPTH-DISCRIMINATION PROPERTIES OF SCANNING MICROSCOPES
    HAMILTON, DK
    WILSON, T
    SHEPPARD, CJR
    [J]. OPTICS LETTERS, 1981, 6 (12) : 625 - 626
  • [8] SCANNING MIRROR MICROSCOPE WITH OPTICAL SECTIONING CHARACTERISTICS - APPLICATIONS IN OPHTHALMOLOGY
    KOESTER, CJ
    [J]. APPLIED OPTICS, 1980, 19 (11) : 1749 - 1757
  • [9] THE TANDEM SCANNING REFLECTED LIGHT-MICROSCOPE
    PETRAN, M
    HADRAVSKY, M
    BOYDE, A
    [J]. SCANNING, 1985, 7 (02) : 97 - 108
  • [10] RASBAND W, 1988, IMAGE 1 04, V36
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