SEPARATION OF FRAGMENTS UP TO 570 BASES IN LENGTH BY USE OF 6-PERCENT T-NON-CROSS-LINKED POLYACRYLAMIDE FOR DNA-SEQUENCING IN CAPILLARY ELECTROPHORESIS

被引：63

作者：

BEST, N ^{[1
]}

ARRIAGA, E ^{[1
]}

CHEN, DY ^{[1
]}

DOVICHI, NJ ^{[1
]}

机构：

[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,AB,CANADA

来源：

ANALYTICAL CHEMISTRY | 1994年 / 66卷 / 22期

关键词：

D O I：

10.1021/ac00094a031

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Non-cross-linked polyacrylamide is a very convenient medium for the separation of DNA sequencing fragments in capillary electrophoresis. We demonstrate DNA sequencing with this matrix at an electric field of 200 V/cm and at room temperature. Resolution is observed to decrease exponentially with fragment length. Fragments 570 bases in length generate a resolution of 0.5, which is adequate for sequence identification.

引用

页码：4063 / 4067

页数：5

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