ASCORBATE FREE-RADICAL AS A MARKER OF OXIDATIVE STRESS - AN EPR STUDY

被引：326
作者：
BUETTNER, GR [1 ]
JURKIEWICZ, BA [1 ]
机构：
[1] UNIV IOWA,COLL MED,RADIAT RES LAB,IOWA CITY,IA 52242
来源：
FREE RADICAL BIOLOGY AND MEDICINE | 1993年 / 14卷 / 01期
关键词：
FREE RADICAL; ANTIOXIDANT; ASCORBATE; ELECTRON PARAMAGNETIC RESONANCE; ASCORBATE RADICAL;
D O I：
10.1016/0891-5849(93)90508-R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The ascorbate free radical (A.-) is a resonance-stabilized tricarbonyl species that is readily for-med from the one-electron oxidation of ascorbate, AH-. Because of the low reduction potential of the A.-/AH- couple, E-degrees' = +282 mV, nearly every oxidizing radical that can arise in a biological system will bring about the one-electron oxidation of AH-, forming A.-. Thus, the steady-state concentration of A.- can be used as a measure of oxidative stress in chemical, biochemical, and biological systems. In this article, we discuss the energetics of ascorbate free radical reactions and demonstrate that the A.- electron paramagnetic resonance (EPR) signal intensity can serve as a marker for the degree of oxidative stress occurring in a system.
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页码：49 / 55
页数：7
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