A PERMSELECTIVE-MEMBRANE ELECTRODE FOR THE ELECTROCHEMICAL STUDY OF REDOX PROTEINS - APPLICATION TO CYTOCHROME C(552) FROM THIOBACILLUS-FERROOXIDANS

被引：61

作者：

HALADJIAN, J ^{[1
]}

BIANCO, P ^{[1
]}

NUNZI, F ^{[1
]}

BRUSCHI, M ^{[1
]}

机构：

[1] CNRS, CHIM BACTERIENNE LAB, F-13277 MARSEILLE 9, FRANCE

来源：

ANALYTICA CHIMICA ACTA | 1994年 / 289卷 / 01期

关键词：

PERMSELECTIVE-MEMBRANE ELECTRODE; CYTOCHROME C; CYTOCHROME C(552); THIOBACILLUS FERROOXIDANS;

D O I：

10.1016/0003-2670(94)80002-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A simple and effective procedure for electrode construction, based on casting a permselective membrane onto a gold electrode surface is described. A redox protein solution is entrapped between the permselective membrane and the electrode surface. The so-mounted permselective-membrane electrode placed in an electrolyte solution is used to study horse heart cytochrome c as a test system. The measured formal potential and the pH dependence profile are in accordance with the literature data. The permselective-membrane electrode is also used to investigate the electrochemistry of cytochrome C-552 from Thiobacillus ferrooxidans and the pH dependence of its formal potential. The performance of this electrode makes it very attractive for studying redox proteins present in limited amounts only.

引用

页码：15 / 20

页数：6

共 2 条

[1] Characterization and expression of the co-transcribed cyc1 and cyc2 genes encoding the cytochrome c4 (c552) and a high-molecular-mass cytochrome c from Thiobacillus ferrooxidans ATCC33020
Appia-Ayme, C
Bengrine, A
Cavazza, C
Giudici-Orticoni, MT
Bruschi, M
Chippaux, M
Bonnefoy, V
FEMS MICROBIOLOGY LETTERS, 1998, 167 (02) : 171 - 177
[2] Application of a large assembly of microelectrodes to the study of cytochrome-c and other redox proteins
Bianco, P
Haladjian, J
Fletcher, S
ELECTROANALYSIS, 1997, 9 (04) : 307 - 310

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