CATION AND SEQUENCE EFFECTS ON STABILITY OF INTERMOLECULAR PYRIMIDINE-PURINE-PURINE TRIPLEX

被引:99
作者
MALKOV, VA
VOLOSHIN, ON
SOYFER, VN
FRANKKAMENETSKII, MD
机构
[1] RUSSIAN ACAD SCI,INST MOLEC GENET,46 KURCHATOV SQ,MOSCOW 123182,RUSSIA
[2] GEORGE MASON UNIV,MOLEC GENET LAB,FAIRFAX,VA 22030
关键词
D O I
10.1093/nar/21.3.585
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A differential effect is found of various bivalent cations (Ba2+, Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+, Zn2+ and Hg2+) on stability of intermolecular Py-Pu-Pu triplex with different sequence of base triads. Ca2+, Mg2+, Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ do stabilize the d(C)n d(G)n d(G)n triplex whereas Ba2+ and Hg2+ do not. Ba2+, Ca2+, Mg2+ and Hg2+ destabilize the d(TC)n d(GA)n d(AG)n triplex whereas Cd2+, Co2+, Mn2+, Ni2+ and Zn2+ stabilize it. The complexes we observe are rather stable because they do not dissociate during time of gel electrophoresis in the co-migration experiments. Chemical probing experiments with dimethyl sulfate as a probe indicate that an arbitrary homopurine-homopyrimidine sequence forms triplex with corresponding purine oligonucleotide in the presence of Mn2+ or Zn2+, but not Mg2+. In the complex the purine oligonucleotide has antiparallel orientation with respect to the purine strand of the duplex. Specifically, we have shown the formation of the Py-Pu-Pu triplex in a fragment of human papilloma virus HPV-16 in the presence of Mn2+.
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页码:585 / 591
页数:7
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