PRIMARY STRUCTURE OF THE 2 VARIANTS OF XENOPUS-LAEVIS MTSSB, A MITOCHONDRIAL-DNA BINDING-PROTEIN

被引：29

作者：

GHRIR, R

LECAER, JP

DUFRESNE, C

GUERIDE, M

机构：

[1] UNIV PARIS 11,INST GENET & MICROBIOL,BIOL GEN LAB,BATIMENT 400,F-91405 ORSAY,FRANCE

[2] CNRS,PHYSIOL NERVEUSE LAB,SERV COMMUN MICROSEQUENCAGE,F-91198 GIF SUR YVETTE,FRANCE

来源：

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1991年 / 291卷 / 02期

关键词：

D O I：

10.1016/0003-9861(91)90152-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structure of the single-stranded DNA binding protein from Xenopus laevis oocyte mitochondria (mtSSB) has been determined by Edman degradation of the intact molecule and peptides derived from partial α-chymotrypsin proteolysis and enzymatic cleavage with trypsin and endoproteinase Glu-C. The native mtSSB is composed of two related polypeptide chains, mtSSBs and mtSSBr. The sequence of mtSSBs consists of 129 amino acids with a calculated molecular mass of 14,627 Da. Comparison of the first 80 residues of the two chains reveals 91% identity. A high degree of similarity is found between mtSSB and Escherichia coli SSB or F sex factor SSB. © 1991.

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页码：395 / 400

页数：6

相关论文

共 14 条

[1] A DNA-BINDING PROTEIN FROM XENOPUS-LAEVIS OOCYTE MITOCHONDRIA [J].

BARAT, M ;

MIGNOTTE, B .

CHROMOSOMA, 1981, 82 (04) :583-593

[2] EFFECT OF DNA CONFORMATION ON THE TRANSCRIPTION OF MITOCHONDRIAL-DNA [J].

BARATGUERIDE, M ;

DUFRESNE, C ;

RICKWOOD, D .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 183 (02) :297-302

[3] F-SEX FACTOR ENCODES A SINGLE-STRANDED-DNA BINDING-PROTEIN (SSB) WITH EXTENSIVE SEQUENCE HOMOLOGY TO ESCHERICHIA-COLI SSB [J].

CHASE, JW ;

MERRILL, BM ;

WILLIAMS, KR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (18) :5480-5484

[4] REPLICATION OF ANIMAL MITOCHONDRIAL-DNA [J].

CLAYTON, DA .

CELL, 1982, 28 (04) :693-705

[5] STRUCTURAL AND FUNCTIONAL-STUDIES OF THE RAT MITOCHONDRIAL SINGLE-STRAND DNA-BINDING PROTEIN-P16 [J].

HOKE, GD ;

PAVCO, PA ;

LEDWITH, BJ ;

VANTUYLE, GC .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 282 (01) :116-124

[6] ON THE USE OF POLYETHYLENIMINE AS A CARRIER FOR PROTEIN SEQUENCING - COMPARISON WITH POLYBRENE [J].

LECAER, JP ;

ROSSIER, J .

ANALYTICAL BIOCHEMISTRY, 1988, 169 (02) :246-252

[7] RAPID AND SENSITIVE PROTEIN SIMILARITY SEARCHES [J].

LIPMAN, DJ ;

PEARSON, WR .

SCIENCE, 1985, 227 (4693) :1435-1441

[8] THE AMINO-TERMINAL SEQUENCE OF THE XENOPUS-LAEVIS MITOCHONDRIAL SSB IS HOMOLOGOUS TO THAT OF THE ESCHERICHIA-COLI PROTEIN [J].

MAHOUNGOU, C ;

GHRIR, R ;

LECAER, JP ;

MIGNOTTE, B ;

BARATGUERIDE, M .

FEBS LETTERS, 1988, 235 (1-2) :267-270

[9]

MERRILL BM, 1984, J BIOL CHEM, V259, P850

[10] EFFECTS OF THE XENOPUS-LAEVIS MITOCHONDRIAL SINGLE-STRANDED DNA-BINDING PROTEIN ON THE ACTIVITY OF DNA POLYMERASE-GAMMA [J].

MIGNOTTE, B ;

MARSAULT, J ;

BARATGUERIDE, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1988, 174 (03) :479-484