STUDIES ON RIBOSOMAL RNA FROM TETRAHYMENA BY ZONE VELOCITY SEDIMENTATION IN SUCROSE GRADIENTS AND BASE RATIO ANALYSIS

被引：24

作者：

KUMAR, A

机构：

[1] Department of Biology, University of Chicago, Whitman Laboratory, Chicago, IL 60637

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 186卷 / 02期

关键词：

D O I：

10.1016/0005-2787(69)90010-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the isolation of ribosomal RNA (rRNA) from Tetrahymena is described. Purified rRNA, when analyzed on sucrose density gradients zonal centrifugation, showed two main peaks sedimenting at about 25.4 S and 17 S. Base ratio analysis showed low (36 %) G+C mole %. On the basis of the sedimentation rate, as well as the low G+C content, it is suggested that Tetrahymena rRNA is unlike the known forms of either prokaryotes or higher eukaryotes and possibly represents an intermediate form. © 1969.

引用

页码：326 / &

共 26 条

[1] COMPARATIVE SEDIMENTATION RATES OF PLANT BACTERIAL AND ANIMAL RIBOSOMAL RNA [J].