CLONING AND HETEROLOGOUS EXPRESSION OF THE PHENAZINE BIOSYNTHETIC LOCUS FROM PSEUDOMONAS-AUREOFACIENS 30-84

被引：192

作者：

PIERSON, LS ^{[1
]}

THOMASHOW, LS ^{[1
]}

机构：

[1] WASHINGTON STATE UNIV,DEPT PLANT PATHOL,USDA ARS,ROOT DIS & BIOL CONTROL RES UNIT,PULLMAN,WA 99164

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1992年 / 5卷 / 04期

关键词：

D O I：

10.1094/MPMI-5-330

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pseudomonas aureofaciens strain 30-84 suppresses take-all disease of wheat caused by Gaeumannomyces graminis var. tritici. Three antibiotics, phenazine-1-carboxylic acid, 2-hydroxyphenazine-1-carboxylic acid, and 2-hydroxyphenazine, were responsible for disease suppression. Tn5-induced mutants deficient in production of one or more of the antibiotics (Phz-) were significantly less suppressive than the parental strain. Cosmids pLSP259 and pLSP282 from a genomic library of strain 30-84 restored phenazine production and fungal inhibition to 10 different Phz- mutants. Sequences required for production of the phenazines were localized to a segment of approximately 2.8 kilobases that was present in both cosmids. Expression of this locus in Escherichia coli required the introduction of a functional promoter, was orientation-specific, and resulted in the production of all three phenazine antibiotics. These results strongly suggest that the cloned sequences encode a major portion of the phenazine biosynthetic pathway.

引用

页码：330 / 339

页数：10