DETERMINATION OF HEMOGLOBIN THROUGH ITS PEROXIDASE-ACTIVITY ON CHLORPROMAZINE

被引：13

作者：

VAZQUEZ, A

TUDELA, J

VARON, R

GARCIACANOVAS, F

机构：

[1] UNIV MURCIA,FAC BIOL,DEPT BIOQUIM,UNIDAD DOCENTE BIOL,EDIFICIO BIOQUIM,PLANTA 2,ALA C,E-30100 MURCIA,SPAIN

[2] UNIV CASTILLA LA MANCHA,ESCUELA UNIV POLITECN ALBACETE,CATEDRA QUIM,LA MANCHA,SPAIN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 23卷 / 01期

关键词：

HEMOGLOBIN; PEROXIDASE ACTIVITY; ENZYME KINETICS; CHLORPROMAZINE; PHENOTHIAZINE;

D O I：

10.1016/0165-022X(91)90049-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination.

引用

页码：45 / 52

页数：8

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