DETERMINATION OF HEMOGLOBIN THROUGH ITS PEROXIDASE-ACTIVITY ON CHLORPROMAZINE

被引：13
|
作者：
VAZQUEZ, A
TUDELA, J
VARON, R
GARCIACANOVAS, F
机构：
[1] UNIV MURCIA,FAC BIOL,DEPT BIOQUIM,UNIDAD DOCENTE BIOL,EDIFICIO BIOQUIM,PLANTA 2,ALA C,E-30100 MURCIA,SPAIN
[2] UNIV CASTILLA LA MANCHA,ESCUELA UNIV POLITECN ALBACETE,CATEDRA QUIM,LA MANCHA,SPAIN
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 23卷 / 01期
关键词：
HEMOGLOBIN; PEROXIDASE ACTIVITY; ENZYME KINETICS; CHLORPROMAZINE; PHENOTHIAZINE;
D O I：
10.1016/0165-022X(91)90049-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Chlorpromazine is an excellent chromogen for determining micro-quantities of hemoglobin. The oxidation of chlorpromazine by peroxidase activity of hemoglobin is coupled to a non-enzymatic reaction of second-order. Kinetic analysis of the overall system leads to a discussion about the optimal assay conditions. Spectrophotometric progress curves for the accumulation of the chlorpromazine cation radical during the reaction have been obtained, and further analyzed by non-linear regression. The use of a linear calibration curve of the enzymatic reaction rate against hemoglobin concentration is proposed for its determination.
引用
收藏
页码：45 / 52
页数：8
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