RAPID ANTIBODY CAPTURE ASSAY FOR DETECTION OF GROUP-A STREPTOCOCCI USING MONOCLONAL-ANTIBODY AND COLLOIDAL GOLD-MONOSPECIFIC POLYVALENT ANTIBODY CONJUGATE

被引：10
作者：
GUPTA, R
TALWAR, GP
GUPTA, SK
机构：
[1] Gamete Antigen Laboratory, National Institute of Immunology, New Delhi, 110067, Shahid Jeet Singh Marg
来源：
JOURNAL OF IMMUNOASSAY | 1992年 / 13卷 / 03期
关键词：
GROUP-A STREPTOCOCCI; ANTIBODY CAPTURE ASSAY; MONOCLONAL ANTIBODY; ANTIBODY-COLLOIDAL GOLD CONJUGATE;
D O I：
10.1080/15321819208021242
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A rapid one step sensitive and specific antibody capture assay for detection of group-A streptococci from the throat swabs of children is described. Monoclonal antibody either MA-106 or MA-107 specific for group-A streptococci polysaccharide (APS) was used as the capture antibody on nitrocellulose paper and rabbit monospecific polyvalent antibody conjugated with colloidal gold to detect the presence of antigen. The lower detection limit of this assay is 15.6ng APS/ml. The assay is specific for APS and failed to recognize polysaccharides obtained from group-B,-C,-G streptococci as well as Staphylococcus aureus. Antigen extracted from throat swabs of children who were positive for beta-hemolytic plaques (other than group-A streptococci) as seen on blood agar culture gave negative readings, thereby confirming the specificity of the assay for APS.
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页码：441 / 455
页数：15
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