FUNCTIONAL-ORGANIZATION OF THE BACTERIOPHAGE PRD1 GENOME

被引：23

作者：

GRAHN, AM

BAMFORD, JKH

ONEILL, MC

BAMFORD, DH

机构：

[1] UNIV HELSINKI,DEPT GENET,SF-00014 HELSINKI,FINLAND

[2] UNIV HELSINKI,INST BIOTECHNOL,SF-00014 HELSINKI,FINLAND

[3] UNIV MARYLAND,DEPT SCI BIOL,BALTIMORE,MD 21228

来源：

JOURNAL OF BACTERIOLOGY | 1994年 / 176卷 / 10期

关键词：

D O I：

10.1128/JB.176.10.3062-3068.1994

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

PRD1 is a broad-host-range virus that infects Escherichia coli cells. It has a linear double-stranded DNA genome that replicates by a protein-primed mechanism. The virus particle is composed of a protein coat enclosing a lipid membrane. On the basis of this structure, PRD1 is being used as a membrane biosynthesis and structure model. In this investigation, we constructed the transcription map of the 15-kb-long phage genome. This was achieved by a computer search of putative promoters, which were then tested for activity by primer extension and for the capability to promote the synthesis of chloramphenicol acetyltransferase.

引用

页码：3062 / 3068

页数：7

共 37 条

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