ANALYSIS OF THE PROTEIN-KINASE ACTIVITY OF ESCHERICHIA-COLI-CELLS

被引：65
作者：
MANAI, M
COZZONE, AJ
机构：
[1] Department of Molecular Biology, University of Lyon, 69622 Villeurbanne
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 03期
关键词：
D O I：
10.1016/0006-291X(79)91953-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
When growing E. coli cells in a minimal medium containing radioactive orthophosphate, several proteins appear to be significantly labeled after analysis by polyacrylamide gel electrophoresis and autoradiography : at least four in the soluble cellular fraction, five in crude ribosomes and one in salt-washed ribosomes. In all cases, phosphorylation occurs at the level of threonine and serine residues as shown by paper electrophoresis of acid hydrolysates of these proteins. It is concluded that bacteria do contain a protein kinase activity. © 1979.
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页码：819 / 826
页数：8
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