STUDIES OF THE SMALL HEAT-SHOCK PROTEINS OF CAENORHABDITIS-ELEGANS USING ANTIPEPTIDE ANTIBODIES

被引：21

作者：

HOCKERTZ, MK

CLARKLEWIS, I

CANDIDO, EPM

机构：

[1] UNIV BRITISH COLUMBIA,DEPT BIOCHEM,VANCOUVER V6T 1W5,BC,CANADA

[2] UNIV BRITISH COLUMBIA,BIOMED RES CTR,VANCOUVER V6T 1W5,BC,CANADA

来源：

FEBS LETTERS | 1991年 / 280卷 / 02期

关键词：

HEAT SHOCK PROTEIN; CAENORHABDITIS-ELEGANS; ALPHA-CRYSTALLIN; AGGREGATION;

D O I：

10.1016/0014-5793(91)80335-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptides corresponding to selected regions of the 16 kDa small heat shock proteins (hsps) of the nematode C. elegans were synthesized and used to elicit polyclonal antibodies. It was found that these antibodies reacted predominantly with either the 16 kDa or the 18 kDa proteins, suggesting a close structural similarity between these hsps. Western blots of two-dimensional gels revealed extensive heterogeneity in these proteins, probably resulting from post-synthetic modifications. The native structures of both size classes of hsps were found to consist of large complexes of 4-5 x 10(5) Da.

引用

页码：375 / 378

页数：4

共 19 条

[1]

AEBERSOLD RH, 1986, J BIOL CHEM, V261, P4229

[2] IMMUNOFLUORESCENCE LOCALIZATION OF A SMALL HEAT-SHOCK PROTEIN (HSP-23) IN SALIVARY-GLAND CELLS OF DROSOPHILA-MELANOGASTER [J].

ARRIGO, AP ;

AHMADZADEH, C .

MOLECULAR AND GENERAL GENETICS, 1981, 184 (01) :73-79

[3] DYNAMIC CHANGES IN THE STRUCTURE AND INTRACELLULAR LOCALE OF THE MAMMALIAN LOW-MOLECULAR-WEIGHT HEAT-SHOCK PROTEIN [J].

ARRIGO, AP ;

SUHAN, JP ;

WELCH, WJ .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (12) :5059-5071

[4]

BRENNER S, 1974, GENETICS, V77, P71

[5] AUTOMATED CHEMICAL SYNTHESIS OF A PROTEIN-GROWTH FACTOR FOR HEMATOPOIETIC-CELLS, INTERLEUKIN-3 [J].

CLARKLEWIS, I ;

AEBERSOLD, R ;

ZILTENER, H ;

SCHRADER, JW ;

HOOD, LE ;

KENT, SBH .

SCIENCE, 1986, 231 (4734) :134-139

[6] ULTRASTRUCTURAL AND BIOCHEMICAL-ANALYSIS OF THE STRESS GRANULE IN CHICKEN-EMBRYO FIBROBLASTS [J].

COLLIER, NC ;

HEUSER, J ;

LEVY, MA ;

SCHLESINGER, MJ .

JOURNAL OF CELL BIOLOGY, 1988, 106 (04) :1131-1139

[7]

EMMONS SW, 1988, NEMATODE CAENORHABDI, P47

[8] 4 SMALL DROSOPHILA HEAT-SHOCK PROTEINS ARE RELATED TO EACH OTHER AND TO MAMMALIAN ALPHA-CRYSTALLIN [J].

INGOLIA, TD ;

CRAIG, EA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (07) :2360-2364

[9]

JONES D, 1986, J BIOL CHEM, V261, P2006

[10] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

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